Protein Info for Atu3285 in Agrobacterium fabrum C58

Annotation: alpha-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 664 PF01055: Glyco_hydro_31_2nd" amino acids 255 to 556 (302 residues), 138.1 bits, see alignment E=4.7e-44 PF21365: Glyco_hydro_31_3rd" amino acids 566 to 651 (86 residues), 71.4 bits, see alignment E=7e-24

Best Hits

Swiss-Prot: 51% identical to SQASE_ECOLI: Sulfoquinovosidase (yihQ) from Escherichia coli (strain K12)

KEGG orthology group: K01811, putative family 31 glucosidase (inferred from 100% identity to atu:Atu3285)

MetaCyc: 100% identical to sulfoquinovosidase (Agrobacterium fabrum)
RXN-17700 [EC: 3.2.1.199]; 3.2.1.199 [EC: 3.2.1.199]

Predicted SEED Role

"Putative glycosidase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.199

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CEZ0 at UniProt or InterPro

Protein Sequence (664 amino acids)

>Atu3285 alpha-glucosidase (Agrobacterium fabrum C58)
MHFETTKDGFTIAIGNRIILSHSPDKPAFFAGFGEERMDMYRGNFDIEDYVIERTALRHA
EVSGDSVTLSSAPGQAPRLRLTLDGNAIRLTALDETINRLWLRVVAETDEHVWGGGEQMS
YFDMRGRRFPLWTSEPGVGRDKTTEITFKSDVSGKAGGDYYNTNYPQPTWLSSRKYALHV
ETSAYSVFDFRNGDFHEIEIWAVPEKIEFFAGDSFADIVSALSLHFGRQPELPDWVYNGA
IIGLKDGVNSFARLEKIRAAGTKVSGLWCEDWVGLRQTSFGARLFWDWQANDTRYPHLRQ
KIAELADQGIRFLGYVNPYLCVDGPLFPVAESAGYFATDVDGKTALVDFGEFDCGVVDFT
NPAAADWFAEEIIGKNMLDFGLSGWMADFGEYLPIDIKLSNGVDAKLMHNAWPTLWAEVN
AKGVESRGKTGEALFFMRAGFTGVQAHCPLIWGGDQSVDFSRHDGLVTVICGALSSGLMG
NAYHHSDIGGYTSLFGNVRTAELIMRWTEMAAFTPVMRTHEGNRPRDNLQIDQDETVLAH
FARMTAIYVALAPYLKSLSAEAAKTGLPVQRPLFLHYENEPQTYAVQDCYLYGADMLVAP
VWKAGETQRSLYLPGHGEWVHLWSGKRHAGGRDITVETPLGEPAVFYRADSSHHRLFEQL
RTIG