Protein Info for Atu3228 in Agrobacterium fabrum C58

Annotation: DNA polymerase III subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1091 TIGR00594: DNA polymerase III, alpha subunit" amino acids 7 to 991 (985 residues), 548.3 bits, see alignment E=2e-168 PF02811: PHP" amino acids 9 to 134 (126 residues), 59 bits, see alignment E=1.9e-19 PF07733: DNA_pol3_alpha" amino acids 275 to 529 (255 residues), 282.1 bits, see alignment E=1.3e-87 PF17657: DNA_pol3_finger" amino acids 532 to 698 (167 residues), 201.6 bits, see alignment E=1.4e-63 PF14579: HHH_6" amino acids 771 to 867 (97 residues), 73.5 bits, see alignment E=3.2e-24 PF01336: tRNA_anti-codon" amino acids 960 to 1031 (72 residues), 34.9 bits, see alignment 3.1e-12

Best Hits

Swiss-Prot: 100% identical to DNE21_AGRFC: Error-prone DNA polymerase 1 (dnaE2-1) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 100% identity to atu:Atu3228)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UAY9 at UniProt or InterPro

Protein Sequence (1091 amino acids)

>Atu3228 DNA polymerase III subunit alpha (Agrobacterium fabrum C58)
MSAPRYAELQVTTHFSFLRGASSCDELFEQAKNLGIEALGVVDRNSLAAIPRAYEAANNH
GVRLVIGCRLDLDDDLSVLVYPMDRAAYGRLCRLLSVGKKRGGKGKCRLSWDDLVAYGEG
LIVVLLADLADDLCALRLRRLKAAFADRAYMALSLRRRPNDQMRLFELSGMAQAAGVPTV
VTNDVLFHVPERRMLQDVVTCIRHNCTIDEAGFRRERHADRYMKPPEEMHRLFARYPEAL
SRSLEIAKRCKFSLKELVYQYPEERSLPGLTAQQALEKMVWEAVPGRYPNGLPEKVEKAL
HHELDVVGRLQYASYFLTVNAIVRYARSKDILCQGRGSAANSVICFVLGITAIDPALFSN
LVFERFVSENRGEPPDIDVDFEHQRREEVIQWVYDTYGRDKAALCSVVTRYRGRGALRDV
GKVLGLPEDLTKLLSSQVWRWSEGVGEKQVKELNLNMEDRRLKLAFELANQLVGTPRHHS
QHPGGFVLSHDRLDELVPIEPAAMNDRQIIEWDKDDIDIVKFMKMDCLALGMLSCMKRGF
DLLEARTGEKYDLAAMPPDDPATFAMIQKADTLGTFQIESRAQMSMLPRLKPAKFYDLVI
QVAIVRPGPIQGDMVHPYLRRRQGKEPVLYEKPQLENILKKTLGVPLFQEQAMRIAMDCA
DFTADEADQLRRAMATFKNVGTISKFKEKLVTGMVANGYDKEFAERIFKQLEGFGSYGFP
ESHAASFALIAYASSWLKCHHPDIFCTAILNSQPMGFYAPAQIVRDARDHGVEVRPVCVN
NSRFDCTLEPTGKKNDKGEERFAVRLGLRMVKGLSNDHAADIVAARQDRPFASVDDLWRR
AGVPAAALVCLAEADAFLPSLRLARREALWAIKALRDEPLPLFAAAAIRENAVIEELQEP
SVALRPMTDGGEVVQDYGHVGLTLREHPMTFLRRDLSRRRIVTCAEAVRVRDGTWLETAG
LVLVRQRPGSAKGVIFMTLEDETGIANAVLWVKTFEKYRRVVLSAGMVGIYGKIQREGEV
VHLVAHRLTDLSHALASVGERNNAFPLPHGRGDEFHHGMPDDHRAIRKRPPPSNHDDDEV
ERIKVISRNFH