Protein Info for Atu3202 in Agrobacterium fabrum C58

Annotation: RND multidrug efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1044 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 386 (21 residues), see Phobius details amino acids 392 to 413 (22 residues), see Phobius details amino acids 436 to 459 (24 residues), see Phobius details amino acids 472 to 497 (26 residues), see Phobius details amino acids 539 to 556 (18 residues), see Phobius details amino acids 867 to 886 (20 residues), see Phobius details amino acids 893 to 913 (21 residues), see Phobius details amino acids 919 to 942 (24 residues), see Phobius details amino acids 968 to 989 (22 residues), see Phobius details amino acids 1000 to 1023 (24 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1038 (1038 residues), 1519.4 bits, see alignment E=0 PF00873: ACR_tran" amino acids 1 to 1022 (1022 residues), 1222.1 bits, see alignment E=0 PF03176: MMPL" amino acids 339 to 496 (158 residues), 38 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 53% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 100% identity to atu:Atu3202)

MetaCyc: 53% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CEU8 at UniProt or InterPro

Protein Sequence (1044 amino acids)

>Atu3202 RND multidrug efflux transporter (Agrobacterium fabrum C58)
MARFFIDRPIFAWVVAIFIMLAGLLAIPMLPIAQYPNVAPPQISIGTNYPGASPEDIYQS
VTRPIEEELNGVPGLIYFESTSESSGRISINVTFEPGTNIGEAQVEVQNRIARVEPRLPR
SVTQQGLRVEQAGTSFLMMVALTSVDGNTDAIGLGDYLSRNVLGELRRVPGVGSAQLFAT
QRSMRIWMDPDKMLGLSLTSDDVINAIQAQNSQVAAGRIGASPNPVGQQISATVNVQGQL
TSPEEFGSIVLRANPDGSSVRLRDVARVEVGGESYNFSSRLNGKPSAAIGVQLSPTANAM
QTSEGVRATMEELSRYFPQGIEYEIPYDTSPFVKISIEKVIHTLIEAMILVFVVMFVFLQ
NIRYTIIPTLVVPVALLGTCAVMYVSGFSINVLTMFAMVLAIGILVDDAIVVVENVERIM
AEEHLSPKEATRKAMGQISGAIIGITLVLTAVFVPMAFFPGAVGIIYQQFSLTMVVSILF
SGFLALSLTPALCASFLKPIKAGHHEKKGFFGWFNRNFDKASHKYSSSVGGIIKRSGRFM
IIYAALLAGLGWAYMQLPSSFLPNEDQGYLIVDIQAPAEASTERTLQSIQQIEKIFMEEP
AVERVIAISGFSFSGSGQNAGLAFATLKDWSERGPEDSAAAISARINGKLWGLPDAMSFA
LSPPPIQGLGNSSGFTFRLQDRSGAGQTALSAAGAQLMAAARQSPVLAGLRIEGMPDAAQ
VNLIIDREKANTFGVTFSDINATISANMGSSYVNDFPNAGRMQRVTVQAEQGQRMKTEDL
LNLNVRNANGGMVPVSSFATVEWVRGPSQVVGYNGYPAIRVSGQSAPGYSSGDAIAEMER
LARELPGGFGFEWTGQSLQEIQSGSQAPALIGLSVLFVFLLLAALYESWSIPLSVMLVVP
LGVIGSVAAVMLRGMPNDVYFLVGLVAIIGLSAKNAILIIEFAKDLRAEGKSTYDATVEA
AHLRFRPILMTSLAFSLGVLPMAIASGASAASQNAIGTGVLGGMISATILAIFFVPVFFV
FVMKFFGDRKKDDKIAADAVSPAE