Protein Info for Atu2770 in Agrobacterium fabrum C58

Annotation: acetoacetyl CoA reductase; poly-beta-hydroxybutyrate biosynthesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01829: acetoacetyl-CoA reductase" amino acids 3 to 240 (238 residues), 366.2 bits, see alignment E=4.3e-114 PF00106: adh_short" amino acids 3 to 191 (189 residues), 202.6 bits, see alignment E=6.7e-64 PF08659: KR" amino acids 5 to 170 (166 residues), 60 bits, see alignment E=4.5e-20 PF13561: adh_short_C2" amino acids 12 to 237 (226 residues), 211.8 bits, see alignment E=1.7e-66

Best Hits

Swiss-Prot: 78% identical to PHAB_RHIME: Acetoacetyl-CoA reductase (phaB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00023, acetoacetyl-CoA reductase [EC: 1.1.1.36] (inferred from 100% identity to atu:Atu2770)

MetaCyc: 76% identical to acetoacetyl-CoA reductase subunit (Zoogloea ramigera)
Acetoacetyl-CoA reductase. [EC: 1.1.1.36]; 1.1.1.36 [EC: 1.1.1.36]

Predicted SEED Role

"Acetoacetyl-CoA reductase (EC 1.1.1.36)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 1.1.1.36)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.36

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CWA4 at UniProt or InterPro

Protein Sequence (241 amino acids)

>Atu2770 acetoacetyl CoA reductase; poly-beta-hydroxybutyrate biosynthesis (Agrobacterium fabrum C58)
MSRVALISGGTSGIGAAIARALQAAGYRVAVNYAFTTDRAEAFQKETGIPAFQWDVRDYD
ACVDGITKVEETFGPVEVLVNNAGITRDAMFHKMSPQQWREVIDTNLTGVFNMTHPVWPG
MRERGFGRIVNISSINGQKGQAGQVNYSASKAGDIGFTKALAQEGASRNITVNAICPGYI
GTEMVRAIPEKVLAERIVPQIPVGRLGEPEEIARCVLFLVSDEAGFITGSTMTANGGQYF
A