Protein Info for Atu2766 in Agrobacterium fabrum C58

Annotation: NAD-glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 33 to 166 (134 residues), 54.6 bits, E=3.9e-18
• PF21073: GDH_HM1
  • amino acids 335 to 394 (60 residues), 67.4 bits, see alignment (E = 2.9e-22)
• PF21076: GDH_ACT2
  • amino acids 398 to 486 (89 residues), 74.8 bits, 2.3e-24
• PF21077: GDH_ACT3
  • amino acids 532 to 610 (79 residues), 64.7 bits, 1.8e-21
• PF21078: GDH_HM3
  • amino acids 630 to 694 (65 residues), 91 bits, see alignment (E = 1.5e-29)
• PF05088: Bac_GDH_CD
  • amino acids 712 to 1206 (495 residues), 826.6 bits, E=2.2e-252
• PF21074: GDH_C
  • amino acids 1251 to 1578 (328 residues), 251.8 bits, E=3.9e-78

Best Hits

KEGG orthology group: K00260, glutamate dehydrogenase [EC: 1.4.1.2] (inferred from 100% identity to atu:Atu2766)

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-alanine degradation II (to D-lactate) (3/3 steps found)
• L-glutamate degradation I (1/1 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• methylaspartate cycle (12/19 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (4/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (1/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Sequence Analysis Tools

See A9CHD5 at UniProt or InterPro

Protein Sequence (1585 amino acids)

>Atu2766 NAD-glutamate dehydrogenase (Agrobacterium fabrum C58)
MGYRNNPKREKQIEKARQQAIAENVQFLDPGILYGRASADDIEYYSAEMLAASAAHSFEA
LSHWTGDAPHISITTVEGVSPRDIPVTVLTIIGRNMPFLYDSVMGEVTSSYRGLYLAVHP
ILVRDDEAQGYRLAEPEDDPAENISLIQLHIAPLTPQAASALEERLRFVLTQVQSAYRDW
RPMLTKLDEALDELSKRGSSRRKTERTEAVEFLNWLRNDNFTFLGMRDYTYSGKGKNAKI
ERGDGVGLGSLSDPDVRVLRLGKNAVTTTPEILAFLDGPDFLIVTKANVKSIVHRRAYMD
YIGIKRFDEDGNVIGELRIVGLFTATAYTRSVKQIPLLRAKVADVERHFGFDPNSHSGRI
LQNTLEAYPRDDLFQIETDLLIRFIEQIMELSDRPRVRVLARIDRFDRFVSAIIFVPREE
YNSYVREKIGDYLSRVYDGHISAYYPAFPEGAVARVHFIVGRTEGKTPRIAQDKLEDAVS
DIAARWIDHFVALSEPGAPVLDVDQAYQEAFTPEEAIGDMPDILAAANGEPVRIEFYQQE
GQSDDTLSLKIFHRDGHLPLSRRVPLLENLGFNVISERTFDIGVVSEGEKRDIVLHDMEL
SVAAGTTLDLPHYGPKLEEAFLAAFSGKVDNDNFNRLILACGLNVREVSVLRAYARYLRQ
TGIVYSQEHISETLYKYPDISRNIFALFKAGFDPSIEEKKRVKKLAEIHKAIEAALSGVP
NLDEDRTLRRYVNAIDATLRTNYFQKNADGTPRDLLAFKFDPKHLDGLPDPRPFREIFVY
GTEVEGVHLRFGKVARGGLRWSDRGQDYRTEVLGLVKAQQVKNAVIVPVGAKGGFFPKNL
PAGGSRDEVFNAGREAYKTYIRTLLSITDNIVDDAIVPPADTLRLDGDDPYFVVAADKGT
ATFSDTANGLAHEAGFWLDDAFASGGSAGYDHKKMGITARGAWETVKRHFREMDTDIQTT
PFTVAGVGDMSGDVFGNGMLLSEKIRLIAAFDHRDIFIDPDPDTDKSFAERKRLFELPRS
SWQDYDRSTLSAGAMIISRSEKSVTLTPEAVAAIGLDKSVATPFEIMTAILKAPTDLLWF
GGIGTYIKAAVETNAEVGDRANDPIRVNATELRAKVIGEGANLGITQKGRIAYALAGGRC
NSDAIDNSAGVNSSDVEVNIKIALASAVNSGRLTMPKRNQLLASMTPDVAQLVLRNNYQQ
SLAISLTERLGLANREELGRLMGALEATGQLNRKVETLPNNAEFSERYASGKPLTRPEIG
VLLSYAKLTLFDALVASPLPDEPYLQHLLLDYFPAKMQKNYANDIKAHRLHREIVATALA
NAVVNRGGPGFVQKLADASGLLAADVVKAAVIVEDGFGLKRLWTEVDALDGKVGGQVQNG
LYATITRIFSDASRLYLQTGSAGAAAGDMATEIERLKTAIKTLSPAATKYRRELGVTEID
GVPSGLLEELDTLSLLVYVPEIMRIAESAGTTLARAAESYATVSSTFRVARLLDASQRIT
PADHYESLALLRSQDQISSSRRRIVISALTEYAKEKDPVQAWYAADRVRVNRIVSELGAL
SESGDTNLARLTVAAGLLGDIVQAR