Protein Info for Atu2732 in Agrobacterium fabrum C58

Annotation: hippurate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 TIGR01891: amidohydrolase" amino acids 5 to 364 (360 residues), 387.3 bits, see alignment E=3.8e-120 PF01546: Peptidase_M20" amino acids 63 to 373 (311 residues), 162.2 bits, see alignment E=1.6e-51 PF07687: M20_dimer" amino acids 175 to 272 (98 residues), 39.4 bits, see alignment E=5.2e-14

Best Hits

Swiss-Prot: 43% identical to HIPO_CAMJE: Hippurate hydrolase (hipO) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 100% identity to atu:Atu2732)

MetaCyc: 44% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32, 3.5.1.47

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CHG0 at UniProt or InterPro

Protein Sequence (379 amino acids)

>Atu2732 hippurate hydrolase (Agrobacterium fabrum C58)
MPDVVAIRRHLHRHPEIGLSEFKTSDFIAEQLVEMGYEVTRGLAGTGIVATLRNGDSTRA
LGIRADIDALPIHEETGADYASTHQGVMHACGHDGHTAMLLGAAKIIAERRNFDGTLHLI
FQPAEENFGGARIMIEDGLFERFPCDAVFALHNDPGLPFGQFVLRDGPILAAVDECKITV
RGYGGHGAEPQDAADPIVAGASIIMALQTVVSRNIHPQLSAVVTVGAFHAGMASNVIPET
AEMLLTIRSFDPGVRDELEKRIRAIAEGQAASYGMSVTIDYERGYNATVNHKAETDYVAD
LARRFAGPEKVLEMKRPSMGAEDFAYMLEKRPGCYFFLGTARTDNDPPLHHPKFDFNDEI
LPIGTTFWVDLAEDYLKAR