Protein Info for Atu2726 in Agrobacterium fabrum C58

Annotation: pyruvate carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1153 PF00289: Biotin_carb_N" amino acids 3 to 118 (116 residues), 130.1 bits, see alignment E=2.5e-41 TIGR01235: pyruvate carboxylase" amino acids 5 to 1151 (1147 residues), 2010.1 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 124 to 329 (206 residues), 240 bits, see alignment E=9.7e-75 PF02222: ATP-grasp" amino acids 148 to 302 (155 residues), 39.5 bits, see alignment E=2.3e-13 PF07478: Dala_Dala_lig_C" amino acids 152 to 300 (149 residues), 29 bits, see alignment E=3.7e-10 PF02785: Biotin_carb_C" amino acids 351 to 458 (108 residues), 113.2 bits, see alignment E=3.3e-36 PF00682: HMGL-like" amino acids 539 to 810 (272 residues), 93 bits, see alignment E=1.2e-29 PF02436: PYC_OADA" amino acids 834 to 1033 (200 residues), 256.8 bits, see alignment E=7.5e-80 PF00364: Biotin_lipoyl" amino acids 1085 to 1149 (65 residues), 59 bits, see alignment 1.7e-19 PF13533: Biotin_lipoyl_2" amino acids 1086 to 1124 (39 residues), 32.6 bits, see alignment (E = 2.6e-11)

Best Hits

Swiss-Prot: 52% identical to PYC_BACSU: Pyruvate carboxylase (pyc) from Bacillus subtilis (strain 168)

KEGG orthology group: K01958, pyruvate carboxylase [EC: 6.4.1.1] (inferred from 100% identity to atu:Atu2726)

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CHG1 at UniProt or InterPro

Protein Sequence (1153 amino acids)

>Atu2726 pyruvate carboxylase (Agrobacterium fabrum C58)
MKISKILVANRSEIAIRVFRAANELGIKTVAIWAEEDKLSLHRFKADESYQVGRGPHLAK
DMGPIESYLSIEEVIRVAKLSGADAIHPGYGLLSESPEFVEACNKAGITFIGPTPDTMRQ
LGNKVAARNLAISVDVPVVPATNPLPDDIAEVERMAEEIGYPVMLKASWGGGGRGMRAIR
KKEDLAREVTEAKREAKAAFGKDEVYLEKLVERARHVESQILGDTHGNVVHLFERDCSIQ
RRNQKVVERAPAPYLSEAQRQELAAYSLKIAAATNYIGAGTVEYLMDADTGKFYFIEVNP
RIQVEHTVTEVVTGIDIVKAQIHILEGAAIGTAESGVPKQEDIRLNGHALQCRITTEDPE
HNFIPDYGRITAYRSASGFGIRLDGGTSYTGAVITRYYDPLLVKVTAWAPEPDEAISRMD
RALREFRIRGVATNLTFLEAIIGHDSFRNNTYTTRFIDSTPELFAQVKRQDRATKLLTYL
ADVTVNGHPETKGRAKPADKAAKPIVPYIDAPTPDGTKQLLDKLGPKGFADWMRNEKRVL
VTDTTMRDGHQSLLATRVRTHDIARVASVYSKALPQLLSLECWGGATFDVSMRFLTEDPW
ERLSLIREGAPNLLLQMLLRGANGVGYKNYPDNVVKYFVRQAARGGVDLFRVFDCLNWVE
NMRVSMDAIAEENKLCEATICYTGDLLNSARPKYDLKYYTNLAVELEKAGAHIIAVKDMA
GLLKPAAAKVLFKALREATGLPIHFHTHDTSGISAATVLAAVDAGVDAVDAAMDAFSGNT
SQPCLGSIVEALSGSERDTGLDTEWIRRISFYWEAVRNQYAAFESDLKGPASEVYLHEMP
GGQFTNLKEQARSLGLESRWHEVAQAYADANRMFGDIVKVTPSSKVVGDMALMMVSQDLT
VADVENPDREVSFPDSVVSMLKGDLGQSPGGWPEALQKKALKGEKPYTVRPGSLLEDADL
DAERKVIETKLERKVDDFEFASYLMYPKVFTDFALTAETYGPVSVLPTHAYFYGMEDGEE
LFADIERGKTLVIVNQASSGIDDKGMVTVFFEINGQPRRIKVPDRAHGASGSAVRRKAEP
GNASHIGAPMPGVISRVFINQGQEVKAGDVLLSIEAMKMETALHAERDGKIAEVLVKPGD
QIDAKDLLISYAE