Protein Info for Atu2723 in Agrobacterium fabrum C58

Annotation: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase" amino acids 12 to 400 (389 residues), 426.4 bits, see alignment E=4.8e-132 PF04551: GcpE" amino acids 17 to 399 (383 residues), 366.7 bits, see alignment E=5.8e-114

Best Hits

Swiss-Prot: 100% identical to ISPG_AGRFC: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 100% identity to atu:Atu2723)

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P58665 at UniProt or InterPro

Protein Sequence (416 amino acids)

>Atu2723 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (Agrobacterium fabrum C58)
MNSPADYDPKPRRASVAVDVGGVIVGGGAPVVVQSMTNTDTADIDATVRQVAALFQAGSE
MVRITVDRDESAAAVPKIRERLLRLGMDVPLIGDFHYIGHKLLADHPACAEALAKYRINP
GNVGFKDKKDKQFGEIVEMAIRYDKPVRIGVNWGSLDQELLTTLMDRNKEQGFPLSARQV
TREAIVQSALISAELAEEIGLPRNRIILSAKVSQVQDLIAVYSMLSERSNHALHLGLTEA
GMGSKGIVASSAAMGYVLQHGIGDTIRVSLTPEPNGDRTREVQVAQELLQVMGFRQFIPV
VAACPGCGRTTSTVFQELAQNIQNDIRKNMPIWREKYPGVEALNVAVMGCIVNGPGESKH
ADIGISLPGTGESPAAPVFIDGEKALTLRGPNIAADFEALVIDYIEKRFGQKDAAE