Protein Info for Atu2627 in Agrobacterium fabrum C58

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 PF17764: PriA_3primeBD" amino acids 24 to 116 (93 residues), 72.7 bits, see alignment E=5.4e-24 PF04851: ResIII" amino acids 207 to 345 (139 residues), 45.7 bits, see alignment E=2.1e-15 PF00270: DEAD" amino acids 211 to 369 (159 residues), 60.6 bits, see alignment E=4.9e-20 TIGR00595: primosomal protein N'" amino acids 230 to 734 (505 residues), 601.6 bits, see alignment E=5.7e-185 PF18319: Zn_ribbon_PriA" amino acids 456 to 482 (27 residues), 34.4 bits, see alignment (E = 5.2e-12) PF18074: PriA_C" amino acids 643 to 734 (92 residues), 62.4 bits, see alignment E=1.8e-20

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 100% identity to atu:Atu2627)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CWL6 at UniProt or InterPro

Protein Sequence (738 amino acids)

>Atu2627 primosomal protein N' (Agrobacterium fabrum C58)
MAKDSSDLFGALFDPPSPTRTVPVLVPLPAPGPYSYAVPEDMVVETGSVVQVPLGPRQVF
GVVWDDAGEKGVDPKKLRPITKSFECPPLKVEMRRFVDWVAAYTLSPPGLVARMALRAPA
AFDPEPMIEGLRLTEGRPERLTPARERVMELAAEGQGWTKSGLAHAAGVSTSVIDGLVKQ
GVFETVFLPAPPVVAEPDPDYVEPRLEGPQKQAASEIIEDVRKGGFHVSLIDGVTGSGKT
EVYFEAVAETLRQGKQVLILLPEIALTAAFLERFQDRFGAKPAEWHSDLSPRMREKVWRQ
AVTGEIKVVAGARSALFLPFDNLGLIIVDEEHDPAYKQEDRVFYNARDMAVVRARIAEFP
VVLVSATPSVESQVNGSSGRYNTIHLHTRFGDAAMPDLHLVDMRRHPPERGGFLSPVLLR
GIGKTIEKGEQSLLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRNQLQCHQCGHN
EPTPDHCPECGTFDHLVACGPGVERIAEEVEKHFPEARTIVLSSDLMGVKRLRLELEAIV
KGEADIVIGTQLVAKGHNFPLMTLVGIVDADLGLANGDPRAAERTFQLLSQVTGRAGRTG
LKSHGLLQTYQPQHPVMQAIVSGDASAFYEREIVEREKAVLPPFGRLASIIVSADTRAEA
ETHARGLRAAAPQVTGIMLLGPAEAPLALIRGRHRFRLLVHGRRNSDMQSFLRTLLASGP
KERGSVHVQLDIDPQSFL