Protein Info for Atu2569 in Agrobacterium fabrum C58

Annotation: thiamine biosynthesis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 17 to 88 (72 residues), 81.9 bits, E=2.4e-27
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 127 to 575 (449 residues), 705.7 bits, E=9e-217
• PF01964: ThiC_Rad_SAM
  • amino acids 128 to 572 (445 residues), 619 bits, E=3.8e-190

Best Hits

Swiss-Prot: 100% identical to THIC_AGRFC: Phosphomethylpyrimidine synthase (thiC) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 100% identity to atu:Atu2569)

MetaCyc: 76% identical to HMP-P synthase monomer (Caulobacter vibrioides)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis II (7/11 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (5/10 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See Q8UCC9 at UniProt or InterPro

Protein Sequence (607 amino acids)

>Atu2569 thiamine biosynthesis protein (Agrobacterium fabrum C58)
MNIVAKTIPLVVTTGPHAASTKIHKPGILHPHIRVPMREIAVHPTAGEPPVTVYDSSGPY
TDPSHPVLIENGLPRLRHDWVVARGDVEAYEGRHVKPEDNGFATGERLTPEFSVRHQPLK
ATAGKAVTQLAYARAGIITPEMEFIAIRENLGREAAKEKPTRDGESFGAHIPDYVTAEFV
RQEVASGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWAIRWG
ADTVMDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVNGIAEDLNWEVFRDTLIEQA
EQGVDYFTIHAGVRLHYIPLTVNRVTGIVSRGGSIMAKWCLHHHKESFLYEHFEEICDIC
RAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTQIAWARDCQVMIEGPGHVPMHKI
KENMDKQLKTCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLG
LPDRNDVKVGVITYKIAAHAADLAKGHPAAQVRDDALSRARFEFRWEDQFNLSLDPDTAR
SFHDETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGLEAMAARFKEGGELYMPL
PTPASAE