Protein Info for Atu2566 in Agrobacterium fabrum C58

Annotation: thiamin biosynthesis ThiG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 PF05690: ThiG" amino acids 4 to 249 (246 residues), 349.9 bits, see alignment E=3.7e-109

Best Hits

Swiss-Prot: 100% identical to THIG_AGRFC: Thiazole synthase (thiG) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03149, thiamine biosynthesis ThiG (inferred from 100% identity to atu:Atu2566)

Predicted SEED Role

"Thiazole biosynthesis protein ThiG" in subsystem Thiamin biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UCD2 at UniProt or InterPro

Protein Sequence (257 amino acids)

>Atu2566 thiamin biosynthesis ThiG (Agrobacterium fabrum C58)
MLTLYGREVSSRLLLGTARYPSPAVLADAVRASNTDILTISLRREMAGAKKGGQFFELIR
ELDRHILPNTAGCHTAKEAVLTAKMAREVFRTDWIKLEVIGHHDTLQPDVFALVEAAKIL
CDEGFEVFPYTTDDLVVAEKLLEAGCRVLMPWCAPIGSAMGPLNLTALKSMRARFPEVPL
IVDAGIGRPSHAVTVMELGYDAVLLNTAVAGAGDPVGMAEAFARAIEAGHQAYLSGPLEP
RDMAVPSTPVIGTAVFS