Protein Info for Atu2497 in Agrobacterium fabrum C58

Annotation: hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 TIGR03611: pyrimidine utilization protein D" amino acids 1 to 255 (255 residues), 420.8 bits, see alignment E=9.6e-131 PF00561: Abhydrolase_1" amino acids 14 to 119 (106 residues), 77.1 bits, see alignment E=2.6e-25 PF12146: Hydrolase_4" amino acids 15 to 239 (225 residues), 63.4 bits, see alignment E=3.1e-21 PF12697: Abhydrolase_6" amino acids 16 to 247 (232 residues), 59 bits, see alignment E=1.6e-19

Best Hits

Swiss-Prot: 100% identical to RUTD_AGRFC: Putative aminoacrylate hydrolase RutD (rutD) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K09023, protein RutD (inferred from 100% identity to atu:Atu2497)

Predicted SEED Role

"Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway" in subsystem Pyrimidine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CWX3 at UniProt or InterPro

Protein Sequence (261 amino acids)

>Atu2497 hydrolase (Agrobacterium fabrum C58)
MHFEVHGRTDAEAPTILLSSGLGGSSAYWLPQIEALSDHFRIVTYDHRGTGRTGGEVPTE
GGISAMADDVLEIVSALNLEKFHFMGHALGGLIGLDIALRQPRLIDRLVLINAWSKADPH
SGRCFDVRIELLEKSGVDAFVKAQPLFLYPAAWMSEHQERLARDDAHGVAHFQGKTNVLR
RIAALRAFDIDARLGEIGNPVLVVATKDDLLVPYTRSLRLAEGLPQSELCLLDFGAHAVN
ITEPDLFNTRLLQFLLPADQT