Protein Info for Atu2483 in Agrobacterium fabrum C58

Annotation: AcrB/AcrD/AcrF family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1064 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 343 to 362 (20 residues), see Phobius details amino acids 369 to 390 (22 residues), see Phobius details amino acids 395 to 419 (25 residues), see Phobius details amino acids 440 to 462 (23 residues), see Phobius details amino acids 472 to 499 (28 residues), see Phobius details amino acids 548 to 568 (21 residues), see Phobius details amino acids 876 to 896 (21 residues), see Phobius details amino acids 905 to 927 (23 residues), see Phobius details amino acids 933 to 956 (24 residues), see Phobius details amino acids 981 to 1002 (22 residues), see Phobius details amino acids 1010 to 1036 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 3 to 1037 (1035 residues), 1202 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 4 to 1044 (1041 residues), 1214.5 bits, see alignment E=0 PF03176: MMPL" amino acids 300 to 498 (199 residues), 43.3 bits, see alignment E=3.5e-15 PF02355: SecD_SecF_C" amino acids 341 to 499 (159 residues), 25.1 bits, see alignment E=1.7e-09

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 100% identity to atu:Atu2483)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CHS0 at UniProt or InterPro

Protein Sequence (1064 amino acids)

>Atu2483 AcrB/AcrD/AcrF family protein (Agrobacterium fabrum C58)
MNISRFFVDRPVFAGVLSILIVVAGLLGMRALPISEYPEVVPPSVVVRATYPGANPTVIA
ETVATPLEEQINGVEDMLYMGSQATSDGVLTLTVTFKLGTDPDKAQQLVQNRVSQAEPRL
PAEVRALGITTVKSSPDFIMVVNLVSKTDAYDITYLRNYATLNVKDRLARIEGVGQVQVF
GAGDYSMRVWLDPQKVAEHDLAAADVSDAIRQQNVQAAAGVIGASPSPNGVDLQLNVNAQ
GRLTTPEEFGNIIVKSGANGEITRLRDVARIELGAADYTLRSLLDGEPAVAVAVLQAPGS
NAIEIADNVRATMDNLQLAMPDGVKYEIVYDTTKFVRSSIEKVVDTLLEAVGLVVLVVIL
FLQTWRASIIPLIAVPVSIIGTFAVMYAFGFSINALTLFGLVLAIGIVVDDAIVVVENVE
RNIENGLSPRDATYKAMREVSGPIIAIALVLVAVFVPLAFISGLSGQFYRQFALTIAIST
VISAINSLTLSPALAALLLKEHGAPKDWLTRFMDKILGWFFRGFNRAFGAASNGYGKTVG
GLVTRKSLVMIVYMALVAATYGMFTTVPSGFVPAQDKQYLIGFAQLPDGATLDRTESVIK
RMSAIAMETPGVSHAIAFPGLSINGFTIGSNSGIVFAVLDDFEKRKTPELSGGAIAMQLN
QKFAGIQDAFIAMFPPPPVNGLGQTGGFKLQIEDRAGYGYQTLDEAAKAVIAKAYQTPEL
AGIFSSFQINVPQLFADLDRAKAEQLGVSVSDVFQTLQIYLGSLYVNDFNAFGRTYSVRV
QADSQFRAHAEDIGRLKVRSATGQMIPLSTLLKVDATTGPERTNRYNGFLAADINGGPAP
GFSSGQAQAAMEKILAENLPAGIDYEWTDLTYQQILAGNSSIVVFPLALLLVYLVLAAQY
ESLTLPIAIILIVPLGVLAALTGVWLTGGDNNIFTQIGLVVLVGLSAKNAILIVEFAREL
EFEGRTPLQAAVEASRLRLRPILMTSLAFIMGVVPLVVSTGAGAEMRAAMGVAVFSGMIG
VTFFGIFMTPVFYVLVRKLAGNRPLIQHKQEEPANSDYKLEKAG