Protein Info for Atu2227 in Agrobacterium fabrum C58

Annotation: 50S ribosomal protein L25

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 202 PF01386: Ribosomal_L25p" amino acids 8 to 95 (88 residues), 88.8 bits, see alignment E=2.6e-29 TIGR00731: ribosomal protein bL25, Ctc-form" amino acids 8 to 185 (178 residues), 155.8 bits, see alignment E=4.7e-50 PF14693: Ribosomal_TL5_C" amino acids 104 to 187 (84 residues), 95.9 bits, see alignment E=1.5e-31

Best Hits

Swiss-Prot: 100% identical to RL25_AGRFC: 50S ribosomal protein L25 (rplY) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02897, large subunit ribosomal protein L25 (inferred from 100% identity to atu:Atu2227)

Predicted SEED Role

"LSU ribosomal protein L25p" in subsystem Ribosome LSU bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UDA0 at UniProt or InterPro

Protein Sequence (202 amino acids)

>Atu2227 50S ribosomal protein L25 (Agrobacterium fabrum C58)
MSKESYELKAEARERVGKGSSRELRRNGLIPAVIYGDKQAPISIALSTNEVTKRIHAGGF
MTTVGTIDVDGKKIKVLPKDYQLDPVRDFTMHVDFLRVSGNTLVNVEIPVHFENEEKSDI
KIGGVLNIVRHTVEFHCPANDIPEAITVDLSGLKIGDSVHISNVKLPKNITPVIADRDFT
IATIVAPAAGVEEETTEEASEE