Protein Info for Atu2185 in Agrobacterium fabrum C58

Annotation: thioredoxin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 324 PF07992: Pyr_redox_2" amino acids 7 to 302 (296 residues), 168 bits, see alignment E=1.1e-52 TIGR01292: thioredoxin-disulfide reductase" amino acids 7 to 313 (307 residues), 403.6 bits, see alignment E=2e-125 PF00890: FAD_binding_2" amino acids 8 to 42 (35 residues), 20.6 bits, see alignment 8.1e-08 PF13738: Pyr_redox_3" amino acids 84 to 285 (202 residues), 68.3 bits, see alignment E=2.6e-22 PF00070: Pyr_redox" amino acids 149 to 211 (63 residues), 56.3 bits, see alignment E=1.4e-18

Best Hits

Swiss-Prot: 62% identical to TRXB_RICCN: Thioredoxin reductase (trxB) from Rickettsia conorii (strain ATCC VR-613 / Malish 7)

KEGG orthology group: K00384, thioredoxin reductase (NADPH) [EC: 1.8.1.9] (inferred from 100% identity to atu:Atu2185)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.9

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CXN8 at UniProt or InterPro

Protein Sequence (324 amino acids)

>Atu2185 thioredoxin reductase (Agrobacterium fabrum C58)
MSARHTKVLIIGSGPAGYTAAIYAARAMLKPVLIAGMEQGGQLMITTDVENYPGFADPIQ
GPWLMDQMLKQATHVGAEIVSDLVTEVETNVRPFVVRTDSGQVWTADTLIIATGAKAKWL
GIESEQHFQGFGVSACATCDGFFYRNKDVIVVGGGNSAVEEALYLAHIAKSVTVVHRRDS
FRSEKILQERLFAKENVKILWNTEIAEITGAPAKPPMPPSVSGVRLRDTKTGAISDTPID
GVFVAIGHAPAVELFKGKVKLKDNGYMWTAPDSTATDVEGIFAAGDVTDDIYRQAITAAG
MGCMAALEAERYLTAQQPLAVAAE