Protein Info for Atu2168 in Agrobacterium fabrum C58

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 661 TIGR01391: DNA primase" amino acids 3 to 421 (419 residues), 430.2 bits, see alignment E=4e-133 PF01807: Zn_ribbon_DnaG" amino acids 5 to 102 (98 residues), 101.2 bits, see alignment E=7.2e-33 PF08275: DNAG_N" amino acids 127 to 253 (127 residues), 144.4 bits, see alignment E=6e-46 PF13662: Toprim_4" amino acids 262 to 328 (67 residues), 63.9 bits, see alignment E=3.9e-21 PF01751: Toprim" amino acids 264 to 334 (71 residues), 35.4 bits, see alignment E=3.1e-12 PF13155: Toprim_2" amino acids 267 to 352 (86 residues), 69.5 bits, see alignment E=7.8e-23 PF10410: DnaB_bind" amino acids 377 to 423 (47 residues), 20.8 bits, see alignment 1.2e-07

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 100% identity to atu:Atu2168)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CI80 at UniProt or InterPro

Protein Sequence (661 amino acids)

>Atu2168 DNA primase (Agrobacterium fabrum C58)
MRFSNSFLDEIRDRVNISDVIGRRVSWDKKKTNAPRGDYWACCPFHGEKSPSFHCEDRKG
RYHCFGCGVSGDHFRFLTELEGLSFPEAVQQIADMAGISMPQPDPQAEKRERERTSLQDV
MEMATLFFQDQLQTSLGARARAYLRDRGLTGRTIETFRLGYAPDSRNALKEHLASKGVSK
EQMEACGLVVHENVPVSYDRFRDRIMFPILSSREKVIAFGGRAMAADAVAKYLNSNETEL
FHKGNVLYNFARARRASQAAGGTVIAVEGYMDVIALYQAGVENAVAPLGTALTDSQLDLL
WKMSPQPVLCFDGDGAGIRAANRAADLALPHIKPDRSVSFALLPDGKDPDDLVRLEGRAP
FDRVLSEAKPLAAMIWSRETSGVTFDTPEKRAQLESRLRQIVTVIADETVRRFYQQDMRD
RLNGFFQPRFQQGGSQGGNFRRDGGQGAGRGFQRNAPGRGGAMSAPASSISDRLTQSGLV
RGHQTKPSLRESVLAITIVNHPELLLEEYDEIAAIDYENRDLQRLWSTVLTYAAESAADI
SRAGLIDRLSQHGFEPLLKTMDQQVRNARLWTATEQAAMEDAREGYVQALSLHKRTKSLL
WQKRELEREIAEATDDERGTLLVRAIAEVQLEIGRMENQEAIIDGFGVMSGRVKGPAFGH
G