Protein Info for Atu2097 in Agrobacterium fabrum C58

Annotation: phospho-N-acetylmuramoyl-pentapeptide- transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 transmembrane" amino acids 20 to 45 (26 residues), see Phobius details amino acids 71 to 90 (20 residues), see Phobius details amino acids 96 to 114 (19 residues), see Phobius details amino acids 134 to 154 (21 residues), see Phobius details amino acids 174 to 196 (23 residues), see Phobius details amino acids 206 to 225 (20 residues), see Phobius details amino acids 245 to 262 (18 residues), see Phobius details amino acids 269 to 288 (20 residues), see Phobius details amino acids 294 to 317 (24 residues), see Phobius details amino acids 344 to 363 (20 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 38 to 366 (329 residues), 393.9 bits, see alignment E=3.3e-122 PF10555: MraY_sig1" amino acids 68 to 80 (13 residues), 23.7 bits, see alignment (E = 2.4e-09) PF00953: Glycos_transf_4" amino acids 99 to 289 (191 residues), 108.6 bits, see alignment E=3.2e-35

Best Hits

Swiss-Prot: 100% identical to MRAY_AGRFC: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 100% identity to atu:Atu2097)

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UDM5 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Atu2097 phospho-N-acetylmuramoyl-pentapeptide- transferase (Agrobacterium fabrum C58)
MLIWLVELSDKVQLFNLFRYITFRAGAAMFTSALIVFLFGPAIINSLRVRQGKGQPIRAD
GPQTHFKKAGTPTMGGLMILAGILGGSLLWGDLSNVYVVAVLMVTLGFGAIGFYDDYLKV
TKQSDKGFSGKARLGIEFLIAAIAVFFMMKMALASAPHGGTLGSSIAFPFFKEFVINLGY
FFVLFGAFVIVGAGNAVNLTDGLDGLAIVPVMIAAATFGVIAYLAGNAVFANYLQINFVP
GTGELAVIVGAVIGAGLGFLWFNAPPAAIFMGDTGSLALGGLIGSIAVATKHEIVMVIVG
GLFVMETLSVIIQVFWFKRTGRRVFLMAPIHHHFEKKGWTESQVVIRFWIISVGLALLGL
ATLKLR