Protein Info for Atu2053 in Agrobacterium fabrum C58

Annotation: FAD dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 52% identical to LDHD_MOUSE: Probable D-lactate dehydrogenase, mitochondrial (Ldhd) from Mus musculus

KEGG orthology group: K00004, (R,R)-butanediol dehydrogenase / diacetyl reductase [EC: 1.1.1.303 1.1.1.4] (inferred from 100% identity to atu:Atu2053)

Predicted SEED Role

"D-Lactate dehydrogenase, cytochrome c-dependent (EC 1.1.2.4)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.2.4)

MetaCyc Pathways

methylglyoxal degradation VIII (3/3 steps found)
pyruvate fermentation to (R)-acetoin I (3/3 steps found)
superpathway of (R,R)-butanediol biosynthesis (4/5 steps found)
(R,R)-butanediol biosynthesis (1/1 steps found)
(R,R)-butanediol degradation (1/1 steps found)
methylglyoxal degradation VI (1/4 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.4

Use Curated BLAST to search for 1.1.1.303 or 1.1.1.4 or 1.1.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CXZ7 at UniProt or InterPro

Protein Sequence (470 amino acids)

>Atu2053 FAD dependent oxidoreductase (Agrobacterium fabrum C58)
MALKDVVAGKRNEEGIASALAVLKQQLGDKLQTGQAFREQHGHTTTYLTLQAPDGVVFAE
SADDVKAVVKACAQYKVPVIPFGTGSSLEGQVNAPNGGICIDFSRMNRIIEVNAEDLDVT
VEPGVTREDLNVYLRDTGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKDNVLAVTAV
VANGEEIRTARRARKSSAGYDLTRLFVGAEGTLGVITSVTLRLQGIPEKITGGVCTFDSI
KAACDAVIMTIQMGVPVARIELLDEVQVRACNAYSGLNYAEKPTLFVEFHGTEETAALQS
QQFAEIAAECGSVDFRWTGDAAERNKLWKARHDAYWASRALAPHLDGLSTDVCVPISRLA
DCVVETQRDIAENGFLAPIVGHAGDGNFHVLILFDGKDAESVAKTEAFVARLNRRAIAMD
GTCTGEHGIGQGKMSFLAEEAGNALDLMRAIKTSLDPDNIFNPGKLFRLV