Protein Info for Atu2045 in Agrobacterium fabrum C58

Annotation: HFLK protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 372 transmembrane" amino acids 63 to 87 (25 residues), see Phobius details PF12221: HflK_N" amino acids 20 to 67 (48 residues), 29.7 bits, see alignment 4.9e-11 TIGR01933: HflK protein" amino acids 89 to 356 (268 residues), 281.3 bits, see alignment E=3.8e-88 PF01145: Band_7" amino acids 91 to 270 (180 residues), 114.4 bits, see alignment E=6.3e-37

Best Hits

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 100% identity to atu:Atu2045)

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CY01 at UniProt or InterPro

Protein Sequence (372 amino acids)

>Atu2045 HFLK protein (Agrobacterium fabrum C58)
MPWSNQNGGGGPWGGGGGGNNQGGGGGPWGQGPNRPRGGGGGNGGPPDLEEIIRRSQDRF
KNVLPGGFNGGVVAIVVLVVLVFLGIQSIYTVQPDERGVELRFGRPKDEISMPGLHFHLW
PIETVEIVKVTEQQQNIGSRASSSSSSGVMLTGDQNIVNVQFSVLYTVSDPKSYLFNVDA
PAETLQQVSESAMREVVGRRPAQDIFRDNRQAIAADVRSIIQSTMDGYGAGISINAVAIE
DAAPPREVADAFDEVQRAEQDEDRFVQEANQYANQKLGAARGQAAQIVEEANAYKSRVVN
EAEGEAQRFISIYDQYRTAPEVTRQRMFLETMEQVLKGSNKIIIDEKQGVVPYLPLNEIM
RNNPGAAQQGGN