Protein Info for Atu2022 in Agrobacterium fabrum C58

Annotation: NADP-dependent alcohol dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 348 PF08240: ADH_N" amino acids 29 to 143 (115 residues), 79.1 bits, see alignment E=2.2e-26 PF00107: ADH_zinc_N" amino acids 185 to 306 (122 residues), 69.1 bits, see alignment E=3.6e-23

Best Hits

Swiss-Prot: 65% identical to ADHC2_MYCS2: NADP-dependent alcohol dehydrogenase C 2 (adhC2) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: K00002, alcohol dehydrogenase (NADP+) [EC: 1.1.1.2] (inferred from 100% identity to atu:Atu2022)

MetaCyc: 49% identical to sinapyl alcohol dehydrogenase (Populus tremuloides)
Cinnamyl-alcohol dehydrogenase. [EC: 1.1.1.195]

Predicted SEED Role

"Alcohol dehydrogenase (EC 1.1.1.1)" in subsystem Fermentations: Mixed acid or Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 1.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.1, 1.1.1.195

Use Curated BLAST to search for 1.1.1.1 or 1.1.1.195 or 1.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CY20 at UniProt or InterPro

Protein Sequence (348 amino acids)

>Atu2022 NADP-dependent alcohol dehydrogenase (Agrobacterium fabrum C58)
MAIARGYAATDASKPLTPFTFERREPNDDDVVIDIKYAGICHSDIHTVRNEWHNAVYPIV
PGHEIAGVVRAVGSKVTRFKVGDHVGVGCFVDSCVGCATRDVDNEQYMPGLVQTYNSVER
DGKSATQGGYSDHIVVREDYVLSIPDNLPLDASAPLLCAGITLYSPLQHWNAGPGKKVAI
VGMGGLGHMGVKIGSAMGADITVLSQTLSKKEDGLKLGAKEYYATSDASTFEKLAGTFDL
ILCTVSAEIDWNAYLNLLKVNGTMVLLGVPEHAIPVHAFSVIPARRSLAGSMIGSIKETQ
EMLDFCGKHDIVSEIETIGIKDVNEAYERVLKSDVRYRFVIDMASLDA