Protein Info for Atu1870 in Agrobacterium fabrum C58

Annotation: isocitrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00127: isocitrate dehydrogenase, NADP-dependent
  • amino acids 3 to 403 (401 residues), 711.6 bits, E=1.1e-218
• PF00180: Iso_dh
  • amino acids 5 to 391 (387 residues), 229 bits, E=5e-72

Best Hits

Swiss-Prot: 76% identical to IDH_SPHYA: Isocitrate dehydrogenase [NADP] (icd) from Sphingobium yanoikuyae

KEGG orthology group: K00031, isocitrate dehydrogenase [EC: 1.1.1.42] (inferred from 100% identity to atu:Atu1870)

MetaCyc: 72% identical to isocitrate dehydrogenase subunit (Mycobacterium tuberculosis H37Rv)
Isocitrate dehydrogenase (NADP(+)). [EC: 1.1.1.42]

Predicted SEED Role

"Isocitrate dehydrogenase [NADP] (EC 1.1.1.42)" in subsystem TCA Cycle (EC 1.1.1.42)

MetaCyc Pathways

• superpathway of glyoxylate bypass and TCA (12/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• TCA cycle I (prokaryotic) (10/10 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• TCA cycle VII (acetate-producers) (8/9 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• cytosolic NADPH production (yeast) (4/5 steps found)
• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (6/8 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• reductive TCA cycle I (7/11 steps found)
• methylaspartate cycle (12/19 steps found)
• ethene biosynthesis V (engineered) (16/25 steps found)
• mixed acid fermentation (9/16 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glutathione metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.42

Sequence Analysis Tools

See A9CII8 at UniProt or InterPro

Protein Sequence (404 amino acids)

>Atu1870 isocitrate dehydrogenase (Agrobacterium fabrum C58)
MAKIKVANPVVDLDGDEMTRIIWQLIKDKLILPYLDLDIEYYDLSVENRDATNDQVTVDA
AHAIKKHGVGIKCATITPDEQRVEEFGLKQMWKSPNGTIRNILGGVIFREPIICKNVPRL
VPGWTKPIVVGRHAFGDQYKATDFKFPGKGKLTIKFVGEDGTVIEKDVFDAPSAGVALAM
YNLDESIREFARASMMYGLMRKWPVYLSTKNTILKAYDGRFKDIFEEVYQTEFKAKFDEV
GITYEHRLIDDMVASALKWSGGYVWACKNYDGDVQSDTVAQGFGSLGLMTSVLLSPDGRT
VEAEAAHGTVTRHYRQHQKGQETSTNSIASIFAWTRGLAHRAKLDDNAELAKFATTLETV
CVDTVESGFMTKDLALLIGPDQPWLSTTAFLDKIDENLKTAMAA