Protein Info for Atu1746 in Agrobacterium fabrum C58

Annotation: threonyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 PF02824: TGS" amino acids 8 to 63 (56 residues), 28.7 bits, see alignment 2.3e-10 TIGR00418: threonine--tRNA ligase" amino acids 74 to 649 (576 residues), 678 bits, see alignment E=6.7e-208 PF07973: tRNA_SAD" amino acids 172 to 221 (50 residues), 51.6 bits, see alignment 1.5e-17 PF00587: tRNA-synt_2b" amino acids 337 to 548 (212 residues), 164.9 bits, see alignment E=4.2e-52 PF03129: HGTP_anticodon" amino acids 560 to 648 (89 residues), 72.5 bits, see alignment E=5.1e-24

Best Hits

Swiss-Prot: 100% identical to SYT_AGRFC: Threonine--tRNA ligase (thrS) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to atu:Atu1746)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UEL1 at UniProt or InterPro

Protein Sequence (667 amino acids)

>Atu1746 threonyl-tRNA synthetase (Agrobacterium fabrum C58)
MSEAISLTFPDGSVRSYPAGTTGREVAESISKSLAKKAVAIALDGTVRDLSETITDGKIE
IVTREDGRALELIRHDAAHVMAEAVQELWPGTQVTIGPVIENGFYYDFAKNEPFTPEDLP
KIEKRMKEIIQRNKPFTREIWSREKAKEVFAAKGENYKVELVDAIPEGQDLKIYYQGDWF
DLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNAMLSRIYGTAWATQEELDNYLHVLAE
AEKRDHRRLGREMDLFHFQEEGPGVVFWHGKGWRMFQTLTAYMRRRLANTYQEVNAPQVL
DKSLWETSGHWGWYQENMFAVKSAHAFTHPDDKEADQRVFALKPMNCPGHVQIFKHGLKS
YREMPVRLAEFGNVHRYEASGALHGLMRVRGFTQDDAHVFCTEEQMAAECLRINDLILSV
YEDFGFSEIVVKLSTRPEKRVGSDDLWDRAESVMLEVLKTIEEQSGGRIKTGILPGEGAF
YGPKFEYTLKDAIGREWQCGTTQVDFNLPERFGAFYIDQNSEKTQPVMIHRAICGSMERF
LGILIENFAGHMPLWFAPLQVVVATITSDADDYGREVAEALREAGMAVETDFRNEKINYK
VREHSVTKVPVIIVCGRKEAEERTVNIRRLGSQNQTSMSLEEAITSLVDEATPPDVKRKL
AAKKQLA