Protein Info for Atu1733 in Agrobacterium fabrum C58

Annotation: ribonucleoside-diphosphate reductase 2 alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1272 PF08471: Ribonuc_red_2_N" amino acids 31 to 147 (117 residues), 149.7 bits, see alignment E=2.7e-48 TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent" amino acids 126 to 753 (628 residues), 477.3 bits, see alignment E=3.8e-147 PF02867: Ribonuc_red_lgC" amino acids 198 to 752 (555 residues), 354.7 bits, see alignment E=9e-110 amino acids 811 to 913 (103 residues), 55.1 bits, see alignment E=5.7e-19

Best Hits

Swiss-Prot: 100% identical to NRDJ_AGRFC: Vitamin B12-dependent ribonucleotide reductase (nrdJ) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 100% identity to atu:Atu1733)

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UEM4 at UniProt or InterPro

Protein Sequence (1272 amino acids)

>Atu1733 ribonucleoside-diphosphate reductase 2 alpha chain (Agrobacterium fabrum C58)
MRIERRFTKPGQSSYAEIEFRKAVSEIKNPDGSVVFRLADIDVPAQFSQVATDVLAQKYF
RKAGVPKLLKKVEENDVPSFLWRSVADEKALKELPEAERYGSETDARQVFDRLAGTWAYW
GWKGKYFSTEEDASAFKDELAYMLATQRVAPNSPQWFNTGLHWAYGIDGPGQGHFYVDPF
TGKLTKSKSAYEHPQPHACFIQSVEDDLVNEGGIMDLWVREARLFKYGSGTGSNFSYLRG
EGEKLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDADHPDIEAYIDWKVNE
EQKVAALVTGSKIVAKHLKAIMKACVNCEADNGDCFDPAKNPALKREIRAAKKDMVPENY
VKRVIQFAEQGYKDIQFKTYDTDWDSEAYLTVSGQNSNNSVSLKDDFLRAVENDGNWNLT
ARKDGKVMKTLKARDLWEKISHAAWASADPGLHFNTTMNDWHTSPAEGPIRASNPCSEYM
FLDDTACNLASLNLLQFKDAKTKRIDIADYEHAVRLWTVVLEVSVMMAQFPSRQIAERSY
EYRTLGLGYANIGGLLMSSGIPYDSDEGRAIAGALTAIMTGVSYATSAEMAGELGPFPSF
APNRDNMLRVIRNHRRAAHGQSEGYEGLSVNPVALIHADCTDQDLVAHATAAWDKALELG
EKHGYRNAQTTVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRAVPESLR
SLGYSESQIAEIEAYAVGHGNLNQAPAINPSTLKAKGFTDEKIEAVNAALKSAFDIKFVF
NQWTLGADFLKGTLKVSDEQLSDMSFNLLDHLGFAKKDIEAANVHVCGAMTLEGAPFLKN
EHLAVFDCANPCGKIGKRYLSVESHIRMMAAAQPFISGAISKTINMPNDATVEDCGAAYM
LSWKLALKANALYRDGSKLSQPLNASLVEDEDDEDFVEELIQQPLAQQAVTITEKIVERV
IERVSREREKLPNRRQGYTQKATVGGHKVYLRTGEFGDGRIGEIFIDMHKEGAAFRAMMN
NFAIAISLGLQYGVPLEEYVEAFTFTKFEPAGMVQGNDAIKNATSILDYVFRELAVSYLG
RHDLAHVDTSDFSNTALGKGIQEGKTNLLSTGWTRGYKPTLVSSNEGDRAASEPKGSATA
APARGSANVTSFAGSAARKLEPTVAITTSEIVSFKRDYEERAKELAEEIAEEVIDEVVQE
AQQTATALFSDKAAADAASAKAEAKKKENERRMRSIAQGYTGNMCSECQNFTMVRNGTCE
KCDTCGATSGCS