Protein Info for Atu1680 in Agrobacterium fabrum C58

Annotation: peptidyl prolyl cis-trans isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 189 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00160: Pro_isomerase" amino acids 29 to 185 (157 residues), 145.1 bits, see alignment E=1.1e-46

Best Hits

Swiss-Prot: 67% identical to PPI1_BRUME: Probable peptidyl-prolyl cis-trans isomerase (ppi) from Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)

KEGG orthology group: None (inferred from 98% identity to agr:AGROH133_06557)

MetaCyc: 53% identical to glucuronan lyase (Sinorhizobium meliloti Rm2011)
Glucuronan lyase. [EC: 4.2.2.14]

Predicted SEED Role

"Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.2.1.8)

Isozymes

Compare fitness of predicted isozymes for: 5.2.1.8

Use Curated BLAST to search for 4.2.2.14 or 5.2.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CIS3 at UniProt or InterPro

Protein Sequence (189 amino acids)

>Atu1680 peptidyl prolyl cis-trans isomerase (Agrobacterium fabrum C58)
MKLVRFAFAGALFAGALAASTFASAAELLTVQLKDGPVVIELMPQVAPKHVAQIEALAKK
GAYDNVAFHRVIDGFMAQTGDVQYGNMEKGFNAQRAGTGGSDMADIPAEFSKTPFTRGVV
GMARSSDPNSANSQFFIMFADGPFLNGQYTVVGKVVSGMEAVDKIKRGAGGNGEVSNPDR
MIKVTVGKK