Protein Info for Atu1622 in Agrobacterium fabrum C58

Annotation: DNA gyrase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 710 TIGR01055: DNA topoisomerase IV, B subunit" amino acids 71 to 702 (632 residues), 743.8 bits, see alignment E=7.8e-228 PF02518: HATPase_c" amino acids 97 to 241 (145 residues), 61.5 bits, see alignment E=2.1e-20 PF00204: DNA_gyraseB" amino acids 297 to 465 (169 residues), 150.1 bits, see alignment E=8.8e-48 PF01751: Toprim" amino acids 493 to 593 (101 residues), 63.4 bits, see alignment E=3.8e-21 PF00986: DNA_gyraseB_C" amino acids 636 to 699 (64 residues), 84.5 bits, see alignment E=9.3e-28

Best Hits

Swiss-Prot: 73% identical to PARE_BARBK: DNA topoisomerase 4 subunit B (parE) from Bartonella bacilliformis (strain ATCC 35685 / NCTC 12138 / KC583)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 100% identity to atu:Atu1622)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CIU8 at UniProt or InterPro

Protein Sequence (710 amino acids)

>Atu1622 DNA gyrase subunit B (Agrobacterium fabrum C58)
MMDDSNDLFSGLPAAQNSDTETNEDTVKPVKAPATAAANVPANRNVQPKPAAIAAIPTSA
PPAPVSPSSDDYGASSIRVLEGLEPVRMRPGMYIGGTDEKALHHLFAEVIDNSMDEAVAG
HANFIEVHLDAEGFLTVTDNGRGIPVENHPQVPGKSTLEVIMTKLHAGGKFDGKAYETSG
GLHGVGVSVVNALSDLLEVEVARNRKLYRQRFSRGAPIGGLEELGDVHNRRGTRVRFHPD
PQIFGDHAKFEPARIFRMARSKAYLFGGVEIRWSCDPGMVPADGEIPEKAVFHFPGGLKD
YLAATLGKEFTVTREIFSGRTEKTGGHGALEWAVTWYGGDTQIHSYCNTIPTPEGGTHEA
GFRIALTKGLKNYAELTQNKRAREITTDDVMISAAGMLSVFIREPEFVGQTKDKLATVEA
QRIVENALRDPFDHYLTGNPGEAAKLLDWVIERAEERLRRRKEKEVNRKTAVRKLRLPGK
LADCSQNTAENAELFIVEGDSAGGSAKQARNRANQAILPLRGKILNVGSASREKLSANQQ
IADLIQALGCGTRTKYREEDLRYERIIIMTDADVDGAHIASLLITFFYQEMPELIRGNHL
YLAVPPLYVIRQGAKSAYARDDAHRAELMETVFKGKKVEIGRFKGLGEMMAAQLKETTMD
PEKRTLLRVEIDDVDFEGTREAVDNLMGTKADARFRFIQERAAFADNLDI