Protein Info for Atu1530 in Agrobacterium fabrum C58

Annotation: nitrogen fixation protein FixG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 527 transmembrane" amino acids 51 to 69 (19 residues), see Phobius details amino acids 103 to 122 (20 residues), see Phobius details amino acids 177 to 195 (19 residues), see Phobius details amino acids 210 to 230 (21 residues), see Phobius details amino acids 384 to 403 (20 residues), see Phobius details TIGR02745: cytochrome c oxidase accessory protein CcoG" amino acids 48 to 515 (468 residues), 561 bits, see alignment E=9.7e-173 PF12801: Fer4_5" amino acids 110 to 142 (33 residues), 22.7 bits, see alignment (E = 2.3e-08) PF13746: Fer4_18" amino acids 231 to 335 (105 residues), 131.3 bits, see alignment E=5.5e-42 PF13534: Fer4_17" amino acids 235 to 299 (65 residues), 24.5 bits, see alignment E=1e-08 PF11614: FixG_C" amino acids 398 to 523 (126 residues), 104.7 bits, see alignment E=1.1e-33

Best Hits

Swiss-Prot: 72% identical to FIXG_RHIME: Nitrogen fixation protein FixG (fixG) from Rhizobium meliloti (strain 1021)

KEGG orthology group: None (inferred from 100% identity to atu:Atu1530)

Predicted SEED Role

"Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation" in subsystem Biogenesis of cbb3-type cytochrome c oxidases or Terminal cytochrome C oxidases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CIY9 at UniProt or InterPro

Protein Sequence (527 amino acids)

>Atu1530 nitrogen fixation protein FixG (Agrobacterium fabrum C58)
MNIYRAGQDRPQQPVERLEAEAVNSAKTRKPLYEARKKIFPKRAEGRFRRFKWLVMLITL
GIYYLTPFLRWDRGPYAPDQAVLIDIANRRFYFFFIEIWPQEFFFVAGLLVMAGLGLFLV
TSAVGRAWCGYTCPQTVWVDLFLVVERAIEGDRNARMKLDAAPFTFGKLRKRVLKHAIWL
VIGVLTGGAWIFYFADAPTLAMQFMTGQAPMIAYSTVATLTATTYVFGGLMREQVCTYMC
PWPRIQAAMLDENSLVVTYNDWRGEPRSRHAKKAAAAGESIGDCVDCNACVAVCPMGIDI
RDGQQLECITCALCIDACDGVMDKIGKPRGLIAYATLSEYQSNMALATGNGEYAIRPANV
REEDGSFSKRVRHFNWRIIFRPRTLLYTAIWTAVGIGLLVALVTRERLALNVLHDRNPQY
VLESNGSIRNGYTVRILNMVPQPRIMNLTIEGLPNAVMKINGMPDTAARAFEVTVEPDEA
TTLKVFVTLPGEDVARTAENFEFIVSDTAGHETARYDAVFNAPGVKK