Protein Info for Atu1528 in Agrobacterium fabrum C58

Annotation: nitrogen fixation protein FixI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 763 transmembrane" amino acids 125 to 146 (22 residues), see Phobius details amino acids 157 to 177 (21 residues), see Phobius details amino acids 189 to 211 (23 residues), see Phobius details amino acids 217 to 235 (19 residues), see Phobius details amino acids 371 to 392 (22 residues), see Phobius details amino acids 399 to 427 (29 residues), see Phobius details amino acids 696 to 714 (19 residues), see Phobius details amino acids 720 to 739 (20 residues), see Phobius details PF00403: HMA" amino acids 42 to 105 (64 residues), 34.8 bits, see alignment E=2.7e-12 TIGR01511: copper-translocating P-type ATPase" amino acids 173 to 738 (566 residues), 555.9 bits, see alignment E=2.4e-170 TIGR01525: heavy metal translocating P-type ATPase" amino acids 191 to 738 (548 residues), 523.7 bits, see alignment E=1.4e-160 TIGR01512: cadmium-translocating P-type ATPase" amino acids 221 to 739 (519 residues), 344.7 bits, see alignment E=1.8e-106 TIGR01494: HAD ATPase, P-type, family IC" amino acids 225 to 722 (498 residues), 223.9 bits, see alignment E=6e-70 PF00122: E1-E2_ATPase" amino acids 253 to 433 (181 residues), 149.3 bits, see alignment E=1.4e-47 PF00702: Hydrolase" amino acids 449 to 650 (202 residues), 101.5 bits, see alignment E=1.2e-32

Best Hits

KEGG orthology group: K01552, [EC: 3.6.3.-] (inferred from 100% identity to atu:Atu1528)

Predicted SEED Role

"Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.4)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-, 3.6.3.4

Use Curated BLAST to search for 3.6.3.- or 3.6.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CIZ1 at UniProt or InterPro

Protein Sequence (763 amino acids)

>Atu1528 nitrogen fixation protein FixI (Agrobacterium fabrum C58)
MSCCAPGTEGSLELGEPLNPPSSEELLLASRDLGQGLRQTDLSVPGVYCGACITTIETAL
GRLGQVERARVNLSSKRVAVVWKEEVNGVRTDPADIARAILATGYRIHLFASGQDASYAL
RSQLIRAVALCGFASANIMLLSVSVWSGADAATRDMFHWISAMIAAPALIYGGRFFYQSA
WNALKHGRTNMDVPIALAITLSYAVSLWETIHHGEHAWFDATVSLLFFLLIGRTLDHIMR
DKARSAIAGLARLSPRGATVIDADGVRDYRPLADIEPGMSIAIAAGDRVAVDAVVVCGSS
DLDMSIVNGESAPRRVVAGDSLQAGTLNLTGSLVARVTASAKDSFLSEVIGLMEAAEGGR
ARYRRIADRAASYYSPVVHLLALVTFLGWGIFGGDWKQAMLIAIAVLIITCPCALGLAVP
VVQVVAAGRLFRRGIMVKEGSAMERLSEIDTVLFDKTGTLTVGKPRLVETADVKPAIMAI
AAGLAAHSRHPLSKALHAAYNGALPAYEAVREIPGSGVEAETEAGTYRLGNRRFACPDET
RINNGDARSEVVLSLDGRHLASFGFEDNPRAGAIAALRSLSVRGLAQEIVSGDRAAAVSA
MAQRLGIGNWRADLSPKDKAARCAALAGEGHRVLMVGDGINDAPALAAAHVSMAPATAAD
IGRQAADFVFMQEDLDAVPFAIETSQRAGNLIRQNFALAIGYNIIAVPIAIAGYATPMIA
AIAMSTSSLIVVANALRLAGAADTILPQKEAKSGFTGEGAQLA