Protein Info for Atu1444 in Agrobacterium fabrum C58

Annotation: nitrogen regulation protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 TIGR00737: putative TIM-barrel protein, nifR3 family" amino acids 17 to 324 (308 residues), 342.8 bits, see alignment E=9.6e-107 PF01207: Dus" amino acids 28 to 299 (272 residues), 318.6 bits, see alignment E=2e-99

Best Hits

Swiss-Prot: 79% identical to DUS_RHILP: Probable tRNA-dihydrouridine synthase (dus) from Rhizobium leguminosarum bv. phaseoli

KEGG orthology group: None (inferred from 100% identity to atu:Atu1444)

Predicted SEED Role

"tRNA dihydrouridine synthase B (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJ25 at UniProt or InterPro

Protein Sequence (338 amino acids)

>Atu1444 nitrogen regulation protein (Agrobacterium fabrum C58)
MKDHHLPLPELSEPFSIGSVTIRNRAVLAPMSGVTDLPFRQLAWRYGAGLVVTEMVASRE
LVANRGESWARLKNAGMVPHMVQLAGREAHFMAEAAKIAADNGAGIIDINMGCPAKKVTG
GYSGSALMRDPDHALSLIEATVGAVDVPVTLKMRLGWDENSINAPEIARRAEAAGVQLIT
IHGRTRMQFYEGRADWDAIRAVREVISVPLIANGDVETAEDAREILRRSGADAVMVGRGA
QGQPWLPAVLAGHSAPQRADIPTIAVEHYEMMLEFYGREAGLRHARKHLGWYLDRSAPDI
ATPEKAQIMTSRDTGEVADLLRSALSENAGEEAARKAA