Protein Info for Atu1436 in Agrobacterium fabrum C58

Annotation: lipoic Acid Synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to LIPA_AGRFC: Lipoyl synthase (lipA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 100% identity to atu:Atu1436)

MetaCyc: 46% identical to lipoyl synthase (Homo sapiens)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

lipoate biosynthesis and incorporation I (2/2 steps found)
lipoate biosynthesis and incorporation III (Bacillus) (2/3 steps found)
lipoate biosynthesis and incorporation V (mammals) (2/3 steps found)
lipoate biosynthesis and incorporation IV (yeast) (4/7 steps found)
lipoate biosynthesis and incorporation II (1/3 steps found)

KEGG Metabolic Maps

Lipoic acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UFG1 at UniProt or InterPro

Protein Sequence (323 amino acids)

>Atu1436 lipoic Acid Synthetase (Agrobacterium fabrum C58)
MVTILDRTSSDEKRIRHPEKAHRPDTEVMRKPEWIRVKAPTSKGYHETRELVRSHKLVTV
CEEAGCPNIGECWEKKHATFMIMGEICTRACAFCNVATGKPNALDMDEPENVAKAVKQMG
LSHVVITSVDRDDLADGGAEHFEKVIWAIRAASPTTTIEILTPDFLKKPGALERVVAAKP
DVFNHNMETVPGNYLTVRPGARYFHSVRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRSADVDFLTIGQYLQPTRKHHKVEAFVTPEEFKSYETVAYAKGFLMVSSSPLTRSS
HHAGDDFERLRAAREKKLLAAAE