Protein Info for Atu1424 in Agrobacterium fabrum C58

Annotation: 2-dehydro-3-deoxyphosphooctonate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 PF00793: DAHP_synth_1" amino acids 17 to 272 (256 residues), 237.8 bits, see alignment E=5.5e-75 TIGR01362: 3-deoxy-8-phosphooctulonate synthase" amino acids 25 to 280 (256 residues), 405 bits, see alignment E=5.5e-126

Best Hits

Swiss-Prot: 100% identical to KDSA_AGRFC: 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01627, 2-dehydro-3-deoxyphosphooctonate aldolase (KDO 8-P synthase) [EC: 2.5.1.55] (inferred from 100% identity to atu:Atu1424)

MetaCyc: 48% identical to 3-deoxy-8-phosphooctulonate synthase subunit (Arabidopsis thaliana col)
3-deoxy-8-phosphooctulonate synthase. [EC: 2.5.1.55]

Predicted SEED Role

"2-Keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase (EC 2.5.1.55)" in subsystem KDO2-Lipid A biosynthesis (EC 2.5.1.55)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.55

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UFH3 at UniProt or InterPro

Protein Sequence (281 amino acids)

>Atu1424 2-dehydro-3-deoxyphosphooctonate aldolase (Agrobacterium fabrum C58)
MSVTNNLKVTAGDGASKVSFCNTERFSLIAGPCQMESRDHAFMIAGVLKELCDSLGIGLV
YKSSFDKANRTSLSGKRGIGLDSAMEIFADLKKEFGFPVLSDIHTEEQCAIVSEVVDVLQ
IPAFLSRQTDLLVAAAKTGRVINVKKGQFLAPWDMKNVLAKLNESGNPNVLLCERGASFG
YNTLVSDMRSLPIMASLGAPVIFDATHSVQQPGGQGGSTGGQREFVETLARAAVAVGVAG
LFIETHEDPDNAPSDGPNMVHLKDMPKLLEKLLAFDAITKA