Protein Info for Atu1380 in Agrobacterium fabrum C58

Annotation: zinc metallopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 transmembrane" amino acids 7 to 28 (22 residues), see Phobius details amino acids 117 to 140 (24 residues), see Phobius details amino acids 297 to 321 (25 residues), see Phobius details amino acids 351 to 370 (20 residues), see Phobius details PF02163: Peptidase_M50" amino acids 18 to 362 (345 residues), 235.7 bits, see alignment E=8.1e-74 TIGR00054: RIP metalloprotease RseP" amino acids 145 to 375 (231 residues), 183.1 bits, see alignment E=4.3e-58 PF00595: PDZ" amino acids 147 to 199 (53 residues), 26 bits, see alignment 1.9e-09 PF13180: PDZ_2" amino acids 147 to 208 (62 residues), 41 bits, see alignment E=3.9e-14 PF17820: PDZ_6" amino acids 148 to 201 (54 residues), 52.4 bits, see alignment 7.3e-18

Best Hits

Swiss-Prot: 100% identical to Y1380_AGRFC: Putative zinc metalloprotease Atu1380 (Atu1380) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K11749, regulator of sigma E protease [EC: 3.4.24.-] (inferred from 100% identity to atu:Atu1380)

Predicted SEED Role

"Membrane-associated zinc metalloprotease" in subsystem Sex pheromones in Enterococcus faecalis and other Firmicutes

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UFL7 at UniProt or InterPro

Protein Sequence (377 amino acids)

>Atu1380 zinc metallopeptidase (Agrobacterium fabrum C58)
MNTIMAATGFLTGYIVPFILVLSLLVFVHEMGHYLVGRWCGIRSTAFSIGFGPELIGFTD
KRGTRWKLSAIPLGGYVKFFGDEDAASKSDSSGLSHMSLEERAQTLSGAKLWKRAATVAA
GPIANFILAILIFAVLFGIYGRMIADPVVAEVRENSAAATAGVKPGDRLVAIDGEKVMTF
EDVRRYVGIRPGTPITVTVERAGEELKLPMVPTRTETTDQFGNKLEMGIIGIVTDQTSGN
FRHIEYSPSEAVAEGVRETGHVITGTFNYIGNLVTGRMNADQLGGPVRVAQASGQMATLG
ISAVIQLAAVLSVSIGLLNLMPVPVLDGGHLVFYAIEAIRGRPLGAGAQEVAFRIGMMMI
LGLMVFATWNDISSLIG