Protein Info for Atu1342 in Agrobacterium fabrum C58

Annotation: peptide chain release factor 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 TIGR00020: peptide chain release factor 2" amino acids 2 to 328 (327 residues), 487.6 bits, see alignment E=1.1e-150 PF03462: PCRF" amino acids 2 to 184 (183 residues), 191 bits, see alignment E=2.1e-60 PF00472: RF-1" amino acids 193 to 302 (110 residues), 152.3 bits, see alignment E=5e-49

Best Hits

Swiss-Prot: 71% identical to RF2_MAGSA: Peptide chain release factor 2 (prfB) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K02836, peptide chain release factor 2 (inferred from 100% identity to atu:Atu1342)

Predicted SEED Role

"Peptide chain release factor 2; programmed frameshift-containing"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJ76 at UniProt or InterPro

Protein Sequence (342 amino acids)

>Atu1342 peptide chain release factor 2 (Agrobacterium fabrum C58)
MNNKAEDPTLWNDASEAQKLMRERQQLDDSINGVKALEQQLKDNIELIELGEMEGDDEIV
KDAEDALKALRSEANRRQVEAMLSGEADSNDTYVEVHSGAGGTESQDWANMLLRMYTRWA
ERQGFKVEVLEIHDGDEAGIKSATILVKGHNAFGWMKTESGVHRLVRISPYDSNARRHTS
FSSIWVYPVVDDSIQIDINESDCRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIAVACQQ
ERSQHKNRAKAWDMLRARLYEVELQKREEAANAQAASKTDIGWGHQIRSYVLQPYQLVKD
LRTGVESTAPGNVLDGDLNEFMEAALAHRISGGADVEVSDID