Protein Info for Atu1330 in Agrobacterium fabrum C58

Annotation: acetyl-CoA carboxylase, biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit" amino acids 1 to 443 (443 residues), 720.8 bits, see alignment E=3.2e-221 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 152.3 bits, see alignment E=1.6e-48 PF02786: CPSase_L_D2" amino acids 115 to 320 (206 residues), 260.3 bits, see alignment E=3.1e-81 PF02222: ATP-grasp" amino acids 123 to 290 (168 residues), 37.9 bits, see alignment E=3.8e-13 PF07478: Dala_Dala_lig_C" amino acids 141 to 289 (149 residues), 43.2 bits, see alignment E=8.2e-15 PF02785: Biotin_carb_C" amino acids 334 to 439 (106 residues), 136.2 bits, see alignment E=1.2e-43

Best Hits

Swiss-Prot: 62% identical to ACCC_HAEIN: Biotin carboxylase (accC) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to atu:Atu1330)

MetaCyc: 61% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJ83 at UniProt or InterPro

Protein Sequence (448 amino acids)

>Atu1330 acetyl-CoA carboxylase, biotin carboxylase (Agrobacterium fabrum C58)
MVSKILIANRGEIALRVLRAAKELGIPTVAVHSTADADAMHVRLADESVCIGPPPSRDSY
LNIHQIVAACEITGADAVHPGYGFLSENAKFADILDAHGITFIGPTAEHIRIMGDKITAK
QTAQELGIPVVPGSDGEVKPENALEIARTIGFPVLIKATAGGGGRGMKVAKTEADLEDAV
ATARSEAAAAFGNDAVYMEKYLGKPRHIEVQVFGDGEGNAVHLGERDCSLQRRHQKVLEE
ANSPALTVEQRMKIGEICADAMRKLKYRGAGTIEFLYENGEFYFIEMNTRLQVEHPVTEA
ITGMDLVQEQIRVASGQGLSVTQADIEFHGHAIECRINAEDPRTFVPSPGTLTYFHTPGG
LGVRVDSGAYQGYKIPPYYDSMIGKLIVHGRDRDECIRRLRRALDEFVVDGIKTTLPLFQ
DLLQNEDILNGDYDIHWLEKYLAGDASH