Protein Info for Atu1330 in Agrobacterium fabrum C58

Annotation: acetyl-CoA carboxylase, biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 110 (110 residues), 152.3 bits, E=1.6e-48
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 443 (443 residues), 720.8 bits, E=3.2e-221
• PF02786: CPSase_L_D2
  • amino acids 115 to 320 (206 residues), 260.3 bits, E=3.1e-81
• PF02222: ATP-grasp
  • amino acids 123 to 290 (168 residues), 37.9 bits, E=3.8e-13
• PF07478: Dala_Dala_lig_C
  • amino acids 141 to 289 (149 residues), 43.2 bits, E=8.2e-15
• PF02785: Biotin_carb_C
  • amino acids 334 to 439 (106 residues), 136.2 bits, E=1.2e-43

Best Hits

Swiss-Prot: 62% identical to ACCC_HAEIN: Biotin carboxylase (accC) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to atu:Atu1330)

MetaCyc: 61% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• jadomycin biosynthesis (3/9 steps found)
• glyoxylate assimilation (5/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (8/18 steps found)
• superpathway of mycolate biosynthesis (22/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See A9CJ83 at UniProt or InterPro

Protein Sequence (448 amino acids)

>Atu1330 acetyl-CoA carboxylase, biotin carboxylase (Agrobacterium fabrum C58)
MVSKILIANRGEIALRVLRAAKELGIPTVAVHSTADADAMHVRLADESVCIGPPPSRDSY
LNIHQIVAACEITGADAVHPGYGFLSENAKFADILDAHGITFIGPTAEHIRIMGDKITAK
QTAQELGIPVVPGSDGEVKPENALEIARTIGFPVLIKATAGGGGRGMKVAKTEADLEDAV
ATARSEAAAAFGNDAVYMEKYLGKPRHIEVQVFGDGEGNAVHLGERDCSLQRRHQKVLEE
ANSPALTVEQRMKIGEICADAMRKLKYRGAGTIEFLYENGEFYFIEMNTRLQVEHPVTEA
ITGMDLVQEQIRVASGQGLSVTQADIEFHGHAIECRINAEDPRTFVPSPGTLTYFHTPGG
LGVRVDSGAYQGYKIPPYYDSMIGKLIVHGRDRDECIRRLRRALDEFVVDGIKTTLPLFQ
DLLQNEDILNGDYDIHWLEKYLAGDASH