Protein Info for Atu1285 in Agrobacterium fabrum C58

Annotation: metal dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 PF00753: Lactamase_B" amino acids 32 to 166 (135 residues), 44.9 bits, see alignment E=3.4e-15 PF12706: Lactamase_B_2" amino acids 35 to 169 (135 residues), 51.6 bits, see alignment E=2.3e-17 PF22505: RNase_J_b_CASP" amino acids 226 to 348 (123 residues), 123.9 bits, see alignment E=8.5e-40 PF07521: RMMBL" amino acids 355 to 410 (56 residues), 41.2 bits, see alignment 3.3e-14 PF17770: RNase_J_C" amino acids 456 to 555 (100 residues), 48.8 bits, see alignment E=3e-16

Best Hits

Swiss-Prot: 80% identical to RNJ_SINM2: Ribonuclease J (rnj) from Sinorhizobium meliloti (strain Sm2011 / Rm2011 / 2011)

KEGG orthology group: K07021, (no description) (inferred from 100% identity to atu:Atu1285)

Predicted SEED Role

"Metallo-beta-lactamase family protein, RNA-specific" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CZK7 at UniProt or InterPro

Protein Sequence (555 amino acids)

>Atu1285 metal dependent hydrolase (Agrobacterium fabrum C58)
MAKQDELVFVPLGGVGEIGMNLALYGYGPESKRQWIMVDCGVTFAGPDLPGVDLVLPDIS
YIAEQKKNLKGIIITHAHEDHYGAMNDLWPGLNVPVYASPFTAGMLEAKRAYEGTRAEIP
VTIFKAGDRINVGPFEIEAVGVNHSIPEPMSLIIRTPLGNVIHTGDWKIDEAPSLGPLTD
EARFRAIGEEGVLALMCDSTNALRDGVSPSEHEVSEGLRQIIESAEGRVAVTTFSSNVGR
IRSIAQAAEAAGREVLLLGSSLKRVVNVSQDLGIMEGIKPFLAEDEYGYIPRDRVVVILT
GSQGEPRAALAKLSRDEMRNVALASGDTVVFSSRAIPGNEKAIIEIKNGLIEQGVHIVTD
NEALVHVSGHPRRNELLRMYEWTKPQILVPVHGEAAHLVAQKELAEQAGISQVPKVRNGN
VLRLAPGPAEVINDVPHGRVFKDGNLVGDMDEMGISNRRKLSFAGHVAVNVVLDSRYDFL
GDPDVVPFGLPEFDDEDEAMEDTLYDAVLGAVESIPRARRKDLALLRESVRRAVRAAANQ
SWGKKPIVTVFVTKV