Protein Info for Atu1269 in Agrobacterium fabrum C58

Annotation: NADH ubiqionone oxidoreductase chain B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to NUOB_AGRFC: NADH-quinone oxidoreductase subunit B (nuoB) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00331, NADH dehydrogenase I subunit B [EC: 1.6.5.3] (inferred from 100% identity to atu:Atu1269)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
aerobic respiration I (cytochrome c) (3/4 steps found)
aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
NAD(P)/NADPH interconversion (3/6 steps found)
Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJB2 at UniProt or InterPro

Protein Sequence (201 amino acids)

>Atu1269 NADH ubiqionone oxidoreductase chain B (Agrobacterium fabrum C58)
MEEGSAGMGMSQNNSTLVAPQPKGIIDPATGKPVGSNDAYFTEINDELADKGFLVTSTDE
LITWARTGSLMWMQFGLACCAVEGLMQASGPRYDMERFGVAPRASPRQSDVMIVAGTLTN
KMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRVVPVDIYVPGCPPTAE
ALLYGVLLLQKKIRRTGTIER