Protein Info for Atu1258 in Agrobacterium fabrum C58

Annotation: ATP-dependent Clp protease, proteolytic subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 210 transmembrane" amino acids 69 to 85 (17 residues), see Phobius details amino acids 94 to 113 (20 residues), see Phobius details TIGR00493: ATP-dependent Clp endopeptidase, proteolytic subunit ClpP" amino acids 9 to 199 (191 residues), 313.4 bits, see alignment E=2.9e-98 PF00574: CLP_protease" amino acids 21 to 200 (180 residues), 290.2 bits, see alignment E=3.3e-91

Best Hits

Swiss-Prot: 100% identical to CLPP2_AGRFC: ATP-dependent Clp protease proteolytic subunit 2 (clpP2) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01358, ATP-dependent Clp protease, protease subunit [EC: 3.4.21.92] (inferred from 100% identity to agr:AGROH133_05606)

MetaCyc: 68% identical to ATP-dependent Clp protease proteolytic subunit (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92)" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent or cAMP signaling in bacteria (EC 3.4.21.92)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UFY6 at UniProt or InterPro

Protein Sequence (210 amino acids)

>Atu1258 ATP-dependent Clp protease, proteolytic subunit (Agrobacterium fabrum C58)
MKNPVDTAMALVPMVVEQTNRGERSYDIFSRLLKERIIFLTGPVEDQMASLVCAQLLFLE
AENPKKEIALYINSPGGVVTAGMAIYDTMQFIRPAVSTLCVGQAASMGSLLLAAGEKGMR
FATPNARIMVHQPSGGFQGQASDIERHARDIIKMKRRLNEVYVKHTGRTLEEVEKTLDRD
HFMDADEAKDWGVIDKILTSRQEMEGAVAN