Protein Info for Atu1195 in Agrobacterium fabrum C58

Annotation: heavy-metal transporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 836 transmembrane" amino acids 167 to 186 (20 residues), see Phobius details amino acids 206 to 225 (20 residues), see Phobius details amino acids 241 to 260 (20 residues), see Phobius details amino acids 271 to 289 (19 residues), see Phobius details amino acids 424 to 445 (22 residues), see Phobius details amino acids 451 to 473 (23 residues), see Phobius details amino acids 767 to 786 (20 residues), see Phobius details amino acids 792 to 813 (22 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 13 to 75 (63 residues), 41.4 bits, see alignment 3.3e-14 amino acids 79 to 142 (64 residues), 45.6 bits, see alignment 1.6e-15 PF00403: HMA" amino acids 14 to 72 (59 residues), 61.7 bits, see alignment 1.7e-20 amino acids 81 to 140 (60 residues), 59.5 bits, see alignment 8.5e-20 TIGR01511: copper-translocating P-type ATPase" amino acids 221 to 817 (597 residues), 604.9 bits, see alignment E=4.1e-185 TIGR01525: heavy metal translocating P-type ATPase" amino acids 239 to 816 (578 residues), 641.7 bits, see alignment E=3.1e-196 TIGR01494: HAD ATPase, P-type, family IC" amino acids 278 to 785 (508 residues), 311.3 bits, see alignment E=2.3e-96 PF00122: E1-E2_ATPase" amino acids 307 to 407 (101 residues), 112.3 bits, see alignment E=2.3e-36 PF00702: Hydrolase" amino acids 505 to 721 (217 residues), 121.8 bits, see alignment E=1.3e-38 PF12710: HAD" amino acids 616 to 717 (102 residues), 30.8 bits, see alignment E=9.8e-11 PF08282: Hydrolase_3" amino acids 695 to 753 (59 residues), 24.8 bits, see alignment 4.2e-09

Best Hits

Swiss-Prot: 70% identical to ATCU_SINMW: Copper-transporting P-type ATPase (actP) from Sinorhizobium medicae (strain WSM419)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to atu:Atu1195)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJE3 at UniProt or InterPro

Protein Sequence (836 amino acids)

>Atu1195 heavy-metal transporting P-type ATPase (Agrobacterium fabrum C58)
MRMSQMEIRASHQIAIDGMTCASCVGRVEKAIARVPGVLKASVNLATERADISFSGPPDV
PAVIAAVRHAGYGVEEKTVELDIEGMTCASCVGRVEKALKAVSGVSDASVNLATERAAIR
VAGNAASAATLAEAIKRAGYQAKEIVADKAGDAEQDRRAADMRSLKISLAVAVVLTLPVF
VLEMGSHLVPAIHDFVMETVGMRKSWYLQFVLTTLVLFGPGLRFFKKGIPALMRLAPDMN
SLVVLGTAAAWGFSVVATFLPEILPRGTANVYYEAAAVIVTLILLGRFLEARAKGRTSEA
IKRLVGLQAKSARVLRDGETIDVPLQDVRTGDVIVVRPGEKVPVDGLVLNGSSYVDESMI
TGEPVPVTKTEGSEVVGGTVNRNGSFTFRATKVGSDTLIAQIIRMVEEAQADKLPIQALV
DKVTNWFVPAVMLAALVTFAVWFVFGPDPALTFALVNAVAVLIIACPCAMGLATPTSIMV
GTGRAAEMGVLFRRGDALQTLRDADVIAVDKTGTLTLGKPKLVHFNTTQGFDADEVLRLV
ASLENRSEHPIAEAIVEAAKHGGLTLAEAEAFEATPGFGVAATVDGRRVEAGADRFMVKL
GYDVGAFATDAERMGREGQSPLYAAVDGRLAAIIAVADPIKQTTPEAIAALHALGLKVTM
ITGDNRRTAEAIARRLGIDEVVAEVLPDGKVEAVKRLAAGGRRVAFVGDGINDAPALAAA
DVGLAIGTGTDVAIESADVVLMSGDLRGVANAIALSKATIRNIRQNLFWAFAYNAALVPV
AAGILYPVNGVLLSPVLAAGAMALSSVFVLTNALRLKSFRAPLVDRSSDLQLAAAE