Protein Info for Atu1153 in Agrobacterium fabrum C58

Annotation: aspartyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 595 TIGR00459: aspartate--tRNA ligase" amino acids 5 to 590 (586 residues), 602.7 bits, see alignment E=3.4e-185 PF01336: tRNA_anti-codon" amino acids 21 to 104 (84 residues), 64 bits, see alignment E=1.5e-21 PF00152: tRNA-synt_2" amino acids 122 to 564 (443 residues), 361.7 bits, see alignment E=4.3e-112 PF02938: GAD" amino acids 314 to 407 (94 residues), 40.6 bits, see alignment E=3.8e-14

Best Hits

Swiss-Prot: 100% identical to SYDND_AGRFC: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 100% identity to agr:AGROH133_05327)

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UG87 at UniProt or InterPro

Protein Sequence (595 amino acids)

>Atu1153 aspartyl-tRNA synthetase (Agrobacterium fabrum C58)
MHRYRSHTCAALRKSDVGETVRLSGWVHRVRDHGGVLFIDLRDHYGITQVVADPDSPAFK
VAETVRGEWVIRIDGLVKARSEDTINKGMATGEIELYAQEIEVLGVAKELPLPVFGEPEY
PEDVRLKYRFLDLRRETLHRNIVKRTQIISSMRKGMGDLGFAEYTTPILTASSPEGARDF
LVPSRIHEGQFFALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVTQEDVWTTMEPMMTAVFEQFAEGKPVTKQWPRIPYDESIRKYGSDKPDLRNPIVME
AVTEHFDGSGFKVFANMIASNPKVQVWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFW
KEEEGKVAGSGPLAKNIGEERTEALRQQLGLEAGDACFFVAGDPAKFYKFAGEARTRAAD
ELNLIDRDRFEMCWIVDFPFFEYNEEEKKIDFAHNPFSMPQGGMEALEGQDPLSIKAFQY
DAVCNGFEIASGSIRNQSPELMVKAFEKVGLSQSDVEERFGGLYRAFQYGAPPHGGCAFG
IDRVVMLLVGAKNLREITLFPMNQQAQDLLMNAPSPATPTQLRELALRVVPSKKD