Protein Info for Atu1144 in Agrobacterium fabrum C58

Annotation: polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 TIGR03705: polyphosphate kinase 1" amino acids 27 to 702 (676 residues), 942.1 bits, see alignment E=9.3e-288 PF13089: PP_kinase_N" amino acids 28 to 133 (106 residues), 123.8 bits, see alignment E=9.7e-40 PF02503: PP_kinase" amino acids 142 to 318 (177 residues), 182.1 bits, see alignment E=2.2e-57 PF17941: PP_kinase_C_1" amino acids 347 to 511 (165 residues), 251.5 bits, see alignment E=7.6e-79 PF13090: PP_kinase_C" amino acids 518 to 696 (179 residues), 205.5 bits, see alignment E=1.1e-64

Best Hits

Swiss-Prot: 67% identical to PPK1_AZOC5: Polyphosphate kinase (ppk) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to atu:Atu1144)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7CZW1 at UniProt or InterPro

Protein Sequence (732 amino acids)

>Atu1144 polyphosphate kinase (Agrobacterium fabrum C58)
MDAIAQEDFNKVQPVAEVENLWDSPARFINREFSWLQFNRRVLEETLNTDHPLLERLRFL
SISAANLDEFFMVRVAGLEGQVRQKITVRTPDGKTPAEQLEDILKEIDNLQMEQQASLAV
LQQYLAKEEIFIVRPAALSEADRTWLGTEFEERIFPVLTPLSIDPAHPFPFIPNLGFSMG
LQLDSVNGREPMTALLRLPTALDRFVRLPDEKNAIRYITLEDVVGLFIHRLYPGYTVRGF
GTFRIIRDSDIEVEEEAEDLVRFFESALKRRRRGSVIRIETDSEMPPSLRQFVVHELGVP
DNRVAVLPGLLALNTISEIVRAPRDDLKFEPYNARFPERVREHAGDCLAAIREKDMVVHH
PYESFDVVVQFLLQAARDPEVLAIKQTLYRTSNDSPIVRALIDAAEAGKSVTALVELKAR
FDEEANIRWARDLERAGVQVVFGFIELKTHAKMSMVVRREDGKLRTYCHLGTGNYHPITA
KIYTDLSFFTCNPKIAHDMANIFNFITGYGEPEEGMKLAISPYTLRARIVKHINEEIEHA
KRGAPAAIWMKMNSLVDPEIIDTLYRASAAGVEIDLVIRGICCLRPQVAGLSDNIRVKSI
VGRFLEHSRIFCFGNGFGLPSDKALVYIGSADMMPRNLDRRVETLVPLTNPTVHEQVLSQ
IMLGNLIDNQQSYEILADGTSRRIEVRKGEEPFNAQHYFMTNPSLSGRGEALKSSAPKLI
AGLISSRKKQAE