Protein Info for Atu1101 in Agrobacterium fabrum C58

Annotation: guanylate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03263: guanylate kinase
  • amino acids 16 to 194 (179 residues), 238.6 bits, E=1.8e-75
• PF00625: Guanylate_kin
  • amino acids 17 to 195 (179 residues), 163.9 bits, E=3.3e-52
• PF13671: AAA_33
  • amino acids 23 to 164 (142 residues), 27.1 bits, E=4.8e-10

Best Hits

Swiss-Prot: 100% identical to KGUA_AGRFC: Guanylate kinase (gmk) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00942, guanylate kinase [EC: 2.7.4.8] (inferred from 100% identity to atu:Atu1101)

MetaCyc: 45% identical to guanylate kinase (Escherichia coli K-12 substr. MG1655)
Guanylate kinase. [EC: 2.7.4.8]; T(2)-induced deoxynucleotide kinase. [EC: 2.7.4.8, 2.7.4.12, 2.7.4.13]

Predicted SEED Role

"Guanylate kinase (EC 2.7.4.8)" in subsystem MLST or Purine conversions (EC 2.7.4.8)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• pyrimidine deoxyribonucleotide phosphorylation (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)
• dZTP biosynthesis (3/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.8

Use Curated BLAST to search for 2.7.4.12 or 2.7.4.13 or 2.7.4.8

Sequence Analysis Tools

See Q8UGD7 at UniProt or InterPro

Protein Sequence (220 amino acids)

>Atu1101 guanylate kinase (Agrobacterium fabrum C58)
MVPVNNSPVTIARRGLMLVISSPSGAGKSTIARNLLEKDKNISLSVSVTTRPRRQSEIEG
IHYHFISKRDFERMRDGDELLEWAEVHGNFYGTPREPVEAAMAAGRDMLFDIDWQGAEQL
QDKMKADVVSIFILPPTMTELQSRLHRRAEDSEEVIKTRLLNSRAEIEHWRDYDYVILND
DLQAAFEGIEAIVKAERVRRDRRHGMFDFVRSLLEEEPKL