Protein Info for Atu1075 in Agrobacterium fabrum C58

Annotation: amidophosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01134: amidophosphoribosyltransferase
  • amino acids 28 to 480 (453 residues), 480.7 bits, E=3e-148
• PF13522: GATase_6
  • amino acids 92 to 221 (130 residues), 63.3 bits, E=3.7e-21
• PF13537: GATase_7
  • amino acids 108 to 225 (118 residues), 64 bits, E=2.1e-21
• PF00156: Pribosyltran
  • amino acids 302 to 405 (104 residues), 36.2 bits, E=5.7e-13

Best Hits

Swiss-Prot: 93% identical to PUR1_RHIEC: Amidophosphoribosyltransferase (purF) from Rhizobium etli (strain CFN 42 / ATCC 51251)

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 100% identity to atu:Atu1075)

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (5/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

See A9CJI5 at UniProt or InterPro

Protein Sequence (502 amino acids)

>Atu1075 amidophosphoribosyltransferase (Agrobacterium fabrum C58)
MIEPLSHSPFSGSTFKEEIDGDTLHEECGVFGILGHDDASALTALGLHALQHRGQEAAGI
VSFDGKRFHQERHMGLVGDHYTDPATLARLPGSIAIGHTRYSTTGEVAMRNVQPLFAELE
EGGISIAHNGNFTNGLTLRRQIIATGAICQSTSDTEVVLHLIARSRHSSTADRFIDAIRQ
MEGGYSMLAMTRTKLIAARDPIGIRPLVMGELDGKPIFCSETCALDIIGAKFVRDVENGE
VIICEIQPDGSITIDARKPSKPQPERLCLFEYVYFARPDSVVGGRNVYTTRKNMGMNLAK
EAPLEADVVVPVPDGGTPAALGFAQASGIPFEYGIIRNHYVGRTFIEPTQQIRAFGVKLK
HSANRAMIEGKRVVLVDDSIVRGTTSVKIVQMIREAGAKEVHIRVASPMIFHPDFYGIDT
PDADKLLANQYADVDAMAKFIGADSLAFLSIDGLYRAVGGENRNHARPQFTDHYFTGDYP
TRLLDQNGEAMGSKISMLASNG