Protein Info for Atu1013 in Agrobacterium fabrum C58

Annotation: ferredoxin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 4 to 301 (298 residues), 220.6 bits, see alignment E=9.4e-69 PF01262: AlaDh_PNT_C" amino acids 143 to 191 (49 residues), 26.1 bits, see alignment 1.6e-09 PF00070: Pyr_redox" amino acids 147 to 225 (79 residues), 61.8 bits, see alignment E=2.2e-20 PF14759: Reductase_C" amino acids 320 to 403 (84 residues), 102.6 bits, see alignment E=4e-33

Best Hits

Swiss-Prot: 47% identical to CNDC1_SPHSD: Chloroacetanilide N-alkylformylase, ferredoxin reductase component (cndC1) from Sphingomonas wittichii (strain DC-6 / KACC 16600)

KEGG orthology group: K00529, ferredoxin--NAD+ reductase [EC: 1.18.1.3] (inferred from 100% identity to atu:Atu1013)

MetaCyc: 43% identical to carbazole 1,9a-dioxygenase ferredoxin reductase component (Sphingomonas sp. XLDN2-5)
RXN-11511 [EC: 1.14.12.22]

Predicted SEED Role

"Nitrite reductase probable [NAD(P)H] subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.14.12.22 or 1.18.1.3 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJL3 at UniProt or InterPro

Protein Sequence (405 amino acids)

>Atu1013 ferredoxin reductase (Agrobacterium fabrum C58)
MTGRLVIVGAGQAAFALASKLRALKDERPITIIGSEDAYPYQRPPLSKKYLLGEMSFDRL
MFRPEEWYAENNVDIRLSTWVEEIDRAAKTVRMQDGSTLSYDKLALATGAAPRLLPASIG
GDLEGVLTVRDKRDADRLVEEMKPGRRLLVIGGGYIGLEAAAVARKLGLEVTLIEMADRI
LQRVAAKETADIMRGIHQAHGVSIREKTGLVRLVGMDGRVAAAELSDGSMLDVDFVIVGI
GVTPNDRLARESGLDVGNGIVVDALTRTSDADIHAVGDCNMLPWRGQHVRLESVQNAVDQ
AEAAAEVLAGTEAAYEAKPWFWSDQYEVKLQIAGFNLGYDETMLRPGAREGSWSVWYFRD
GRFVAVDAVNDAKAYVAGKKLLDIGAEPDRAILADAAADLKLLLS