Protein Info for Atu0937 in Agrobacterium fabrum C58

Annotation: copper transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 841 transmembrane" amino acids 176 to 196 (21 residues), see Phobius details amino acids 212 to 232 (21 residues), see Phobius details amino acids 248 to 267 (20 residues), see Phobius details amino acids 277 to 296 (20 residues), see Phobius details amino acids 430 to 452 (23 residues), see Phobius details amino acids 458 to 480 (23 residues), see Phobius details amino acids 780 to 802 (23 residues), see Phobius details amino acids 808 to 827 (20 residues), see Phobius details PF00403: HMA" amino acids 1 to 55 (55 residues), 43.9 bits, see alignment 5.2e-15 TIGR01525: heavy metal translocating P-type ATPase" amino acids 246 to 829 (584 residues), 615.3 bits, see alignment E=2.5e-188 TIGR01494: HAD ATPase, P-type, family IC" amino acids 285 to 799 (515 residues), 294.4 bits, see alignment E=2.6e-91 PF00122: E1-E2_ATPase" amino acids 314 to 494 (181 residues), 191.3 bits, see alignment E=2.3e-60 PF00702: Hydrolase" amino acids 513 to 734 (222 residues), 127.3 bits, see alignment E=2e-40

Best Hits

Swiss-Prot: 64% identical to ATCU2_RHIME: Copper-transporting ATPase 2 (actP2) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to atu:Atu0937)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A9CJP7 at UniProt or InterPro

Protein Sequence (841 amino acids)

>Atu0937 copper transporting ATPase (Agrobacterium fabrum C58)
MTCASCVRRVETAAAKVPGVASSSVNFATKKLTVEPAEGFSARTLGAAIKKVGYDIAPER
QEFAVDGLHGEAEAERLKAVLNAVATTVNVKVDAAAGKVAVETIGGRRERDALVETAKLA
GFALKTPKPHGHSAHHDHGQHHAHHQGHSQMTAAGESGGHDHMQHAGEEGALKRDLTIAA
ILTAPLFVLEMGGHIYEPMHHWLMGIIETQNLYYIYFVLATAVIFGPGLRFLKAGFPALL
RGAPEMNSLVALGVTAAYLYSVVATFAPDLLPAEAQFVYYEAATVIVTLILTGRLLEARA
SGRTGDAIRKLMSLQAKTARVERDGATIDISPDDLVAGDIIVIRPGERLAVDGEVVEGSS
YVDESMISGEPVPVEKTVGATVVGGTINKTGAFKFKATKVGADTMLSQIIRMVEEAQGSK
LPIQLLVDRVTALFVPVVIAIAVLTFIVWAIFGPEPAYTFALVNAVAVLIIACPCAMGLA
TPTSIMVGTGRAAELGVLFRKGQALQELRSAQIVVVDKTGTVTKGRPELTDLVVAEGFAD
NEVLALVAAVEGRSEHPIAEAIVRAAEEKNVATPAGLAPTTVENFESVTGYGIAATVNGR
KVEVGADRYMAKLGHSVDIFAEAAARLGDEGKTPLYAAIDGRLAAAIAVADPLKPSSVTA
IKALQAMGIEVAMVTGDNERTANAIARQVGISRVVAEVLPEGKVKAIHEMRAGGKVLAFV
GDGINDAPALAEADIGIAVGTGTDVAIESADVVLVGGDLLGAVNAIEMSRATMRNIKENL
FWAFGYNVALIPVAAGVLYPAFGITLSPMIGAGAMALSSVFVLANALRLKRAKVVHREVT
S