Protein Info for Atu0811 in Agrobacterium fabrum C58

Annotation: malate:quinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to MQO_AGRFC: Probable malate:quinone oxidoreductase (mqo) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 100% identity to atu:Atu0811)

MetaCyc: 68% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

superpathway of glyoxylate bypass and TCA (12/12 steps found)
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
TCA cycle I (prokaryotic) (10/10 steps found)
TCA cycle VII (acetate-producers) (8/9 steps found)
partial TCA cycle (obligate autotrophs) (7/8 steps found)
TCA cycle VI (Helicobacter) (6/9 steps found)

KEGG Metabolic Maps

Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UH73 at UniProt or InterPro

Protein Sequence (544 amino acids)

>Atu0811 malate:quinone oxidoreductase (Agrobacterium fabrum C58)
MGGALAGIAAAALPSAPLLANMAAKKVDVLLIGGGIMSATLGVWLRELEPTWSMQMLERL
DGVALESSNGWNNAGTGHSALAELNYTPEDDNGNIKITQAVNINESFQISRQFWAWQVRN
GVLKNPRSFINHTPHMSFVWGDENVAYLEKRYQALKASPLFAGMEFSTDPEQIKKWVPLM
MEGRDPSQKLGATWSPLGTDMEFGEITRQFVSHLQSDQNFDLQVNSEVSDIQRNADGSWR
VTYTNTKTDAEQVVDAKFVFIGAGGGALHLLQMSGVPEADDYAGFPVGGSFLINENPDVT
MQHLAKAYGKASVGSPPMSVPHLDTRVLGGKRVILFGPFATFSTKFLKEGSYFDLVSSVT
TSNAWPMVRVGIDEYPLVEYLAGQLMMSDDDRFAALKEYFPNAKQGEWRLWQAGQRVQII
KRDEEKGGVLRLGTEVVAAKDGSIAGLLGASPGASTAAPIMLSVLEKVFKDKVATPEWQA
KLRQIVPSYGTKLNDDPEKVREEWAYTAEHLQLPTPPQIDLEALKGAGAAPAGAPVKKVP
DIAL