Protein Info for Atu0803 in Agrobacterium fabrum C58

Annotation: pyruvate,orthophosphate dikinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 908 TIGR01828: pyruvate, phosphate dikinase" amino acids 2 to 884 (883 residues), 1342.6 bits, see alignment E=0 PF01326: PPDK_N" amino acids 21 to 58 (38 residues), 28.3 bits, see alignment (E = 1.6e-10) amino acids 61 to 370 (310 residues), 138 bits, see alignment E=6.9e-44 PF00391: PEP-utilizers" amino acids 436 to 516 (81 residues), 84.5 bits, see alignment E=4.8e-28 PF02896: PEP-utilizers_C" amino acids 531 to 882 (352 residues), 262.8 bits, see alignment E=5.7e-82

Best Hits

Swiss-Prot: 80% identical to PPDK_RHIME: Pyruvate, phosphate dikinase (ppdK) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01006, pyruvate,orthophosphate dikinase [EC: 2.7.9.1] (inferred from 100% identity to atu:Atu0803)

Predicted SEED Role

"Pyruvate,phosphate dikinase (EC 2.7.9.1)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q7D0M6 at UniProt or InterPro

Protein Sequence (908 amino acids)

>Atu0803 pyruvate,orthophosphate dikinase (Agrobacterium fabrum C58)
MKKWVYTFGNGAAEGGAGDVAILGGKGANLAEMASLGLPVPPGLTIIADACALYHKNGRD
LPEELKLQVMAGLHGMEIVTGKTFGGSHSPLLISVRSGARASMPGMMDTVLNLGLNDRTV
EALGHDAGDARFAWDSYRRFIQMYGDVVMGLDHEMFEEILEDEKGRLGHEYDTDMSAVEW
QHVVSLYKKLIEDELGEAFPQDCHVQLWGAIGAVFASWTNHRAVTYRHLHNIPGDWGTAV
NVQAMVFGNLGSSSATGVAFTRNPSTGEAELYGEFLVNAQGEDVVAGIRTPQSITEAARL
VSGSDKPSMEKLMPEAFSEFLAICKRLETHYRDMQDLEFTIERGKLWMLQTRSGKRTTRA
AMKIAVDMVEAGLISQEEAVCRIEPSSLDQLLHPTIDPGISRPIIGSGLPASPGAATGEI
VFTSEEAVAAEAEGRRVILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGIPC
VTGAGSMRVDMRNKVLIGVGCMLKRGDVITIDGSSGRVLKGEVPMTQPELSGDFGKLMQW
ADNLRRMTVRTNADTPADARAARAFGAEGIGLCRTEHMFFEGDRIHVMREMILAESEKGR
RAALDQLLPMQRSDFTELFQIMHGLPVTIRLLDPPLHEFLPKSDGEIVEVAAAMGMPQTV
FRQRLDALHEFNPMLGHRGCRLAISYPEIAEMQARAIFEAAVAAARITGAPVVPEIMVPL
VGLRSELDYVKAVIDTVAAEVAEETGMSIEYLTGTMIELPRAALRAHVIAEAAEFFSFGT
NDLTQTTFGISRDDAARFINTYQRKGIIERDPFISLDFDGVGELIRIAAERGRQTRPELK
LGICGEHGGDPASIHFCEDADLDYVSCSPFRVPIARLAAAQATLAAKAKQGETPSNVAFI
PVRGSVGR