Protein Info for Atu0760 in Agrobacterium fabrum C58

Annotation: pyridoxamine 5'-phosphate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 206 TIGR00558: pyridoxamine 5'-phosphate oxidase" amino acids 5 to 206 (202 residues), 240 bits, see alignment E=1.1e-75 PF12766: Pyridox_oxase_2" amino acids 24 to 107 (84 residues), 40.6 bits, see alignment E=4.9e-14 PF01243: Putative_PNPOx" amino acids 25 to 111 (87 residues), 98.2 bits, see alignment E=3.8e-32 PF10590: PNP_phzG_C" amino acids 164 to 206 (43 residues), 61.9 bits, see alignment 6.5e-21

Best Hits

Swiss-Prot: 100% identical to PDXH_AGRFC: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 100% identity to atu:Atu0760)

MetaCyc: 44% identical to pyridoxine/pyridoxamine 5'-phosphate oxidase (Escherichia coli K-12 substr. MG1655)
Pyridoxal 5'-phosphate synthase. [EC: 1.4.3.5]; 1.4.3.5 [EC: 1.4.3.5]

Predicted SEED Role

"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.3.5

Use Curated BLAST to search for 1.4.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UHC2 at UniProt or InterPro

Protein Sequence (206 amino acids)

>Atu0760 pyridoxamine 5'-phosphate oxidase (Agrobacterium fabrum C58)
MSETGLTSSDFTEENEPFALFAEWLKDATASEINDPNAVALATVDENGLPNVRMVLLKGV
DDRGFVFYTNFESQKGQEILGQKKAAMCFHWKSLRRQVRLRGEVEIVSDEEADAYYASRP
RGSRIGAWASKQSRPLEGRFALEKAVAEYTARYAIGEIPRPSHWSGFRIRPVSIEFWHDR
KFRLHDRIEFRRETPDGAWSKVRMYP