Protein Info for Atu0697 in Agrobacterium fabrum C58
Annotation: tetraacyldisaccharide 4'-kinase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to LPXK_AGRFC: Tetraacyldisaccharide 4'-kinase (lpxK) from Agrobacterium fabrum (strain C58 / ATCC 33970)
KEGG orthology group: K00912, tetraacyldisaccharide 4'-kinase [EC: 2.7.1.130] (inferred from 100% identity to atu:Atu0697)MetaCyc: 51% identical to tetraacyldisaccharide 4'-kinase (Brucella abortus 2308)
Tetraacyldisaccharide 4'-kinase. [EC: 2.7.1.130]
Predicted SEED Role
"Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130)" in subsystem KDO2-Lipid A biosynthesis or Lipopolysaccharide-related cluster in Alphaproteobacteria (EC 2.7.1.130)
MetaCyc Pathways
- lipid IVA biosynthesis (P. gingivalis) (9/9 steps found)
- lipid IVA biosynthesis (2,3-diamino-2,3-dideoxy-D-glucopyranose-containing) (6/6 steps found)
- lipid IVA biosynthesis (E. coli) (6/6 steps found)
- lipid IVA biosynthesis (H. pylori) (6/6 steps found)
- lipid IVA biosynthesis (P. putida) (6/6 steps found)
- lipid IVA biosynthesis (Vibrio cholerae serogroup O1 El Tor) (6/6 steps found)
- lipid IVA biosynthesis (generic) (6/6 steps found)
- superpathway of (Kdo)2-lipid A biosynthesis (11/17 steps found)
- superpathway of Kdo2-lipid A biosynthesis (11/25 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.7.1.130
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8UHI5 at UniProt or InterPro
Protein Sequence (348 amino acids)
>Atu0697 tetraacyldisaccharide 4'-kinase (Agrobacterium fabrum C58) MVSEAPPFWWQKAGWQAWLLSPFSLLYGKVAGRRMRTAKRANVPVPVICIGNFTVGGAGK TPTAIAIARAAVARGMKPGFLSRGYGGTLDVTTLVDAQHHRAAAVGDEPLLLAREAVTVI SRRRVEGAHRLVKEGVNLIIMDDGFQSARLTLDYALVVIDTVRGIGNGHLVPGGPVRAPL AEQMRQMTGLLKVGKGHAADPLVRQAAKAAKPVFVAAIMPQEPEDFRGKRVLAFAGIADP AKFYRTVEALGGDIVLSRSFPDHHHFSDDEIDDLLKDARKENLQLVTTAKDAVRLNGHHG RAEELLWNSQVIEIDMVFDDPNAAGTVIETAVVNCRARLLRDNARSST