Protein Info for Atu0686 in Agrobacterium fabrum C58

Annotation: isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 967 TIGR00392: isoleucine--tRNA ligase" amino acids 21 to 872 (852 residues), 801.7 bits, see alignment E=4.3e-245 PF00133: tRNA-synt_1" amino acids 35 to 678 (644 residues), 439 bits, see alignment E=3.3e-135 PF09334: tRNA-synt_1g" amino acids 64 to 115 (52 residues), 39.8 bits, see alignment 5.2e-14 PF08264: Anticodon_1" amino acids 723 to 872 (150 residues), 93.8 bits, see alignment E=1.9e-30

Best Hits

Swiss-Prot: 100% identical to SYI_AGRFC: Isoleucine--tRNA ligase (ileS) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 100% identity to atu:Atu0686)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UHJ6 at UniProt or InterPro

Protein Sequence (967 amino acids)

>Atu0686 isoleucyl-tRNA synthetase (Agrobacterium fabrum C58)
MSDTAEKLDYSTTLYLPQTDFPMRAGLPQKEPETVKRWQDMGLYKKLRASAAGREKFVLH
DGPPYANGNIHIGHALNKILKDVITRSFQMRGFDANYVPGWDCHGLPIEWKIEEAYRARG
KNKDEVPVNEFRKECRDFAAGWIKVQSEEFKRLGIEGDFENPYTTMNFHAEARIAGELLK
IARSGQLYRGSKPIMWSVVERTALAEAEVEYHDVESDMIWVKFPVKDAAPALSGVSVVIW
TTTPWTIPGNRAIAFSSRVEYGLFEVESAQNDFGPQTGEKLIFARKLADEAAAKAKLTFK
FVRDVKSEELAAITCAHPLASLGYDFPVPLLDGDHVTDDAGTGFVHTAPSHGREDFDAWT
NSVRTLEARGIDTKIPFPVDDAGFYTEDAPGFGPSAEGGAARVMDDNGKKGDANERVIKA
LIAANNLFARGRLKHSYPHSWRSKKPVIFRNTPQWFVYMDKELGDGTTLRSRSLDAIDQT
RFVPASGQNRLRAMIEGRPDWVLSRQRSWGVPIAVFADEQGEVLVDEAVNARILEAFEAE
GADAWFAEGAKERFLGNDHDHAKWQQVMDILDVWFDSGCTHTFTLEDRPDMKWPADVYLE
GSDQHRGWFHSSLLESCATRGRAPYNAVVTHGFTMDEQGRKQSKSLGNVVAPQDVMKESG
ADILRLWVMTTDYWEDQRLGKAIIQTNIDAYRKLRNTVRWMLGTLAHDNGEEIAYADLPE
LEKLVLHRLSELDKVVRDGYDAFDFKKITRALIDFANVELSAFYFDIRKDTLYCDAPSSL
KRRASLSVIAKLFDCLVTWLAPMLPFTTEEAWLSRYPDAESVHLAQFPEIPAEWKNDALE
AKWEKIRKVRTVVTGALEVERREKRIGSSLEAAPVVHIADSDLLAALSGQDFAEICITSA
ITVVGDEGPADGFRLQEVAKVVVEQKLAEGVKCARSWRITTDVGSDPQYPDVSARDAAAL
RELAALK