Protein Info for Atu0685 in Agrobacterium fabrum C58

Annotation: riboflavin biosynthesis bifunctional protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 15 to 169 (155 residues), 175.5 bits, E=7.4e-56
• TIGR00125: cytidyltransferase-like domain
  • amino acids 21 to 50 (30 residues), 27.9 bits, see alignment (E = 2e-10)
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 21 to 311 (291 residues), 248.5 bits, E=9.7e-78
• PF01687: Flavokinase
  • amino acids 188 to 311 (124 residues), 124.2 bits, E=3.9e-40

Best Hits

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to atu:Atu0685)

Predicted SEED Role

"FMN adenylyltransferase (EC 2.7.7.2) / Riboflavin kinase (EC 2.7.1.26)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (3/3 steps found)
• flavin salvage (2/2 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

See A9CK17 at UniProt or InterPro

Protein Sequence (326 amino acids)

>Atu0685 riboflavin biosynthesis bifunctional protein (Agrobacterium fabrum C58)
MTVFHRNEKKEPLPEALRGGVIAIGNFDGVHRGHRAVLDRALELAEARGVPALVLTFEPH
PRSVFRPDTPVFRLTPAPLKARILEAIGFRSVIEYPFDREFSQRSADEFVQSILVDWLGA
SAVVTGFDFHFGKGREGGPAFLMAAGKRHGFDVTLVDAFRDEGSDVVSSSRIRSLLCEGD
VAGAAGLLGYRFTVESEVIGGQKLGRTLGYPTANMALAPETELKAGIYAVRFRRLDGSIH
DGVASFGYRPTVTENGAALLETFLFDFSGDLYGEVCSVSFFGHLRDELKFDGLDPLVAQI
RRDEEEARAMLSGVRPLSQLDAKIAF