Protein Info for Atu0678 in Agrobacterium fabrum C58

Annotation: histidyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 PF13393: tRNA-synt_His" amino acids 12 to 193 (182 residues), 67.2 bits, see alignment E=7.8e-23 amino acids 236 to 366 (131 residues), 52.1 bits, see alignment E=3e-18

Best Hits

Swiss-Prot: 100% identical to HISZ_AGRFC: ATP phosphoribosyltransferase regulatory subunit (hisZ) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K02502, ATP phosphoribosyltransferase regulatory subunit (inferred from 100% identity to atu:Atu0678)

Predicted SEED Role

"ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17)" in subsystem Histidine Biosynthesis (EC 2.4.2.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.17

Use Curated BLAST to search for 2.4.2.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UHK2 at UniProt or InterPro

Protein Sequence (375 amino acids)

>Atu0678 histidyl-tRNA synthetase (Agrobacterium fabrum C58)
MPLIDMPEFAGELLEEFAARRTSRVNTPVIQPAEPFLDMAGEDLRRRIFMTESETGESLC
LRPEFTIPVCLRHIETATGTPKRYSYLGEVFRQRREGASEFYQAGIEDLGDTDIAAADAR
VVIDATAILQRLLPGRSLAVTLGDQQVFEAVVAALGLPLGWQKRLVQAFGDMAQLDALLE
SLVHPKPMTGLDARVAGLLATGDEAVLVDYLDTVMQETGYSTNASRSPLEIARRLREKLA
LAATRLPDESFELLKQFLALQAPLPQASQVLGDFAARAKLKLDGALSAFDKRVAALANAG
VDLETVTYGAAFGRPLDYYTGLVFEVVEAGSDSVLAGGGRYDRLLTLLGAQEKIPAVGFS
LWLDRIKAVRGSDKP